Augmentation of cellular immunity and protection against influenza virus infection by bovine late colostrum in mice

Abstract Objective We investigated whether oral administration of skimmed and concentrated bovine late colostrum (SCBLC) activates the immune system and protects against influenza virus (Flu) infection. Methods Murine Peyer's patch (PP) cells (2.5 105) were cultured in 0.1 ml RPMI-1640 suppleme...

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Bibliographic Details
Published in:Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2012-04, Vol.28 (4), p.442-446
Main Authors: Uchida, Kenji, M.S, Hiruta, Naoyuki, B.S, Yamaguchi, Hiroshi, B.S, Yamashita, Kousaku, M.S, Fujimura, Katsuyuki, B.S, Yasui, Hisako, Ph.D
Format: Article
Language:English
Subjects:
Animal models
Animals
Biological and medical sciences
Body weight
Cattle
Cells
Colostrum - immunology
Cytokines product
Disease Models, Animal
Feeding. Feeding behavior
Female
Fundamental and applied biological sciences. Psychology
Gastroenterology and Hepatology
Immune system
Immunity, Cellular
Infections
Influenza
Influenza Vaccines
Influenza virus
Influenza virus infection
Interferon-gamma - metabolism
Interleukin-12 - metabolism
Killer Cells, Natural
Lung - cytology
Lung - immunology
Mice
Mice, Inbred BALB C
Natural killer cells
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - prevention & control
Peyer's Patches - cytology
Peyer's Patches - immunology
Pregnancy
Proteins
Respiratory tract
Rodents
Skimmed and concentrated bovine late colostrum
Spleen - cytology
Spleen - immunology
Vaccination
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Viruses
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Summary:Abstract Objective We investigated whether oral administration of skimmed and concentrated bovine late colostrum (SCBLC) activates the immune system and protects against influenza virus (Flu) infection. Methods Murine Peyer's patch (PP) cells (2.5 105) were cultured in 0.1 ml RPMI-1640 supplemented with SCBLC at a concentration of 0, 0.1 or 1.0 mg/ml. To determine the levels of IL-12 and IFN-, supernatants were collected on day 3. Mice were orally administered sterile saline solution (control group), or 400 g/g body weight (SCBLC 400 group) or 2,000 g/g body weight (SCBLC 2,000 group) of SCBLC for three weeks. These mice were measured for natural killer (NK) cells activity on PP cells, splenocytes and lung cells. Also, these mice in the control and SCBLC 2,000 groups were infected with Flu and were measured for the accumulated symptom rate. Results In PP cells cultured with SCBLC, the levels of IL-12 and IFN- were significantly increased in vitro. Oral administration of SCBLC to mice significantly increased NK cell activity of PP cells, splenocytes and lung cells. The accumulated symptom rate of the SCBLC 2,000 group was significantly lower than that of the control group in a mouse model of Flu infection. Conclusion These results indicate that oral administration of SCBLC activates not only systemic cellular immunity but also local cellular immunity, such as in the respiratory tract, and that activation of cellular immunity is one of the mechanisms of amelioration of Flu infection.
ISSN:0899-9007
1873-1244
DOI:10.1016/j.nut.2011.07.021