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Photo fermentative hydrogen production using dominant components (acetate, lactate, and butyrate) in dark fermentation effluents
Engineering strategies were applied to promote the phototrophic H 2 production of an indigenous purple nonsulfur bacterium Rhodopseudomonas palustris WP3-5 using major components (i.e., acetate, butyrate, and lactate) of dark fermentation effluents as carbon sources. First, performance of cell growt...
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| Published in: | International journal of hydrogen energy 2011-10, Vol.36 (21), p.14059-14068 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | Engineering strategies were applied to promote the phototrophic H
2 production of an indigenous purple nonsulfur bacterium
Rhodopseudomonas palustris WP3-5 using major components (i.e., acetate, butyrate, and lactate) of dark fermentation effluents as carbon sources. First, performance of cell growth and photo-H
2 production on each carbon source was examined individually. It appeared that acetate was the most effective carbon source for photo-H
2 production, giving an overall H
2 production rate and H
2 yield of 12.68 ml/h/l and 67.1%, respectively. Next, the effect of substrate concentration of each carbon source on photo-hydrogen production was investigated. Kinetic models were developed to describe the correlation between maximum specific growth rate/specific H
2 production rate and the substrate concentration. The results show that using acetate and lactate as the carbon source, the kinetics for the cell growth and photo-hydrogen production can be described by Monod-type and Michaelis–Menten models, respectively, whereas substrate inhibition occurred when using butyrate as the carbon source. The continuous cultures were also conducted at a hydraulic retention time of 96 h using synthetic dark fermentation soluble metabolites (with a 5 and 10 fold dilution) as the influent. The phototrophic H
2 production efficiency was stably maintained for over 30 days with an overall H
2 yield 10.30 and 11.97 mol H
2/mol sucrose, when using 5-fold and 10-fold diluted dark fermentation effluent, respectively, as the substrate for dark fermentation. This demonstrates the feasibility of using the sequential dark and photo fermentation for high-yield biohydrogen production.
► Soluble metabolites from dark fermentation were converted to H
2 by photo fermentation. ► Kinetics of cell growth and H
2 production by
R.
palustris WP3-5 was identified. ► Integration of dark and photo fermentation led to a high yield of 6 mol H
2/mol hexose. |
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| ISSN: | 0360-3199 1879-3487 |
| DOI: | 10.1016/j.ijhydene.2011.04.148 |