Obtaining monoglycerides by esterification of glycerol with palmitic acid using some high activity preparations of Candida antarctica lipase B

► Use of CLEA, PCMC and CLPCMC for monoglyceride synthesis. ► Monopalmitin with ≤10% diglyceride obtained. ► Process optimization resulted in 87% MG in 24h. Cross-linked enzyme aggregates (CLEAs), protein coated microcrystals (PCMCs), cross-linked protein coated microcrystals (CLPCMCs) of Candida an...

Full description

Saved in:
Bibliographic Details
Published in:Process biochemistry (1991) 2012-03, Vol.47 (3), p.503-508
Main Authors: Kapoor, Manali, Gupta, Munishwar N.
Format: Article
Language:English
Subjects:
Candida antarctica
Cross-linked enzyme aggregates
Cross-linked protein coated microcrystals
Crosslinking
Diglycerides
Glycerol esterification
Lipase
Lipases
Microcrystals
Moisture content
Molecular sieves
Monoglyceride synthesis
Monoglycerides
Palmitic acid
Protein coated microcrystals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► Use of CLEA, PCMC and CLPCMC for monoglyceride synthesis. ► Monopalmitin with ≤10% diglyceride obtained. ► Process optimization resulted in 87% MG in 24h. Cross-linked enzyme aggregates (CLEAs), protein coated microcrystals (PCMCs), cross-linked protein coated microcrystals (CLPCMCs) of Candida antarctica lipase B (CALB) were used for esterification of glycerol with palmitic acid in acetone under low water condition. With CLEAs, 81% monoglyceride (MG) along with 4.5% diglyceride (DG) were produced at 1% (v/v) water content in 24h. The water content in the medium was managed by stepwise addition of the molecular sieves at appropriate time intervals. With PCMCs (potassium sulfate as a core material), 82% monoglyceride along with 4.0% diglyceride were obtained, with 0.5% water (v/v) added initially to anhydrous acetone with molecular sieves present in the reaction medium. With CLPCMC (prepared by cross-linking with 200mM glutaraldehyde), 87% monoglyceride and 3.3% diglyceride were produced in 24h in presence of 1% (v/v) water (added initially) and with appropriate amount of molecular sieves added in the reaction medium. The results offer a comparative study on the performance of three high activity preparations of CALB for preparation of monopalmitin with ≤10% of the diglyceride content.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2011.12.009