Administration of antisense DNA for GPR39-1b causes anxiolytic-like responses and appetite loss in rats

► We examined the effect of antisense oligonucleotides for GPR39-1b, which is the receptor for obestatin, on anxiety and food intake. ► We infused GPR39-1b antisense oligonucleotides into the cerebral lateral ventricles of rats. ► The antisense oligonucleotides produced anxiolytic-like effects in th...

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Bibliographic Details
Published in:Neuroscience research 2012-03, Vol.72 (3), p.257-262
Main Authors: Ishitobi, Yoshinobu, Akiyoshi, Jotaro, Honda, Shuhei, Ninomiya, Taiga, Kanehisa, Masayuki, Tanaka, Yoshihiro, Tsuru, Jusen, Isogawa, Koichi, Kitamura, Hirokazu, Fujikura, Yoshihisa
Format: Article
Language:English
Subjects:
Animals
Antisense
Anxiety
Anxiety - genetics
Anxiety - metabolism
Appetite
Appetite - drug effects
Appetite - genetics
Behavior, Animal - drug effects
Body Weight - drug effects
Body Weight - genetics
Dentate Gyrus - drug effects
Dentate Gyrus - metabolism
DNA, Antisense - administration & dosage
Eating - drug effects
Eating - genetics
Emotions - drug effects
GPR39
Hippocampus
Male
Rat
Rats
Rats, Wistar
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
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Summary:► We examined the effect of antisense oligonucleotides for GPR39-1b, which is the receptor for obestatin, on anxiety and food intake. ► We infused GPR39-1b antisense oligonucleotides into the cerebral lateral ventricles of rats. ► The antisense oligonucleotides produced anxiolytic-like effects in the elevated-plus maze test and the black and white test. ► The antisense oligonucleotides decreased food intake. The G protein-coupled receptor 39-b (GPR39-1b) is a splice variant of which is expressed in the central nervous and gastrointestinal systems. Previously, GPR39-1b was proposed to be the receptor for obestatin, but current evidence does not support this hypothesis. The purpose of the present work was to identify the role of GPR39-1b in anxiety and eating behaviors. Antisense oligonucleotides were infused at a constant rate into the cerebral lateral ventricles of rats and their effect on anxiety-like behavior and food intake was monitored. GPR39-1b antisense oligonucleotides produced anxiolytic-like effects in the elevated-plus maze test and in the black and white box test. Antisense oligonucleotides also decreased food intake. These results indicate that inhibition of GPR39-1b induces a decrease in anxiety-related behaviors and disturbs appetite.
ISSN:0168-0102
1872-8111
DOI:10.1016/j.neures.2011.12.002