Quantitation of glucocorticoid receptor alpha and NF-κB pathway mRNA and its correlation with disease activity in rheumatoid arthritis patients

We measured NF-κB, IKK, c-Fos, and GRα mRNA expression and in vivo glucocorticoid sensitivity in patients with rheumatoid arthritis. A very low dose intravenous dexamethasone suppression test and real-time PCR quantitation of mRNA of these genes were performed on blood samples from 21 rheumatoid art...

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Bibliographic Details
Published in:Genetics and molecular research 2010-01, Vol.9 (4), p.2300-2310
Main Authors: Cavalcante, L O, Melo, M R, Dinis, V G, Castro, R B, Souza, B D B, Longui, C A
Format: Article
Language:English
Subjects:
Aged
Antirheumatic Agents - therapeutic use
Arthritis, Rheumatoid - drug therapy
Arthritis, Rheumatoid - genetics
Arthritis, Rheumatoid - pathology
Base Sequence
c-Fos protein
Case-Control Studies
Dexamethasone
Dexamethasone - administration & dosage
DNA Primers
Gene expression
Glucocorticoid receptors
Glucocorticoids
Humans
Hydrocortisone
Hydrocortisone - blood
IKK protein
Intravenous administration
Middle Aged
mRNA
Multivariate analysis
NF- Kappa B protein
NF-kappa B - genetics
Polymerase chain reaction
Quantitation
Receptors, Glucocorticoid - genetics
Reverse Transcriptase Polymerase Chain Reaction
Rheumatoid arthritis
RNA, Messenger - metabolism
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Summary:We measured NF-κB, IKK, c-Fos, and GRα mRNA expression and in vivo glucocorticoid sensitivity in patients with rheumatoid arthritis. A very low dose intravenous dexamethasone suppression test and real-time PCR quantitation of mRNA of these genes were performed on blood samples from 21 rheumatoid arthritis patients who were not on glucocorticoids during the previous four months and on blood samples from 20 healthy individuals. Mean rheumatoid arthritis duration was 8.8 years, and mean disease activity, as assessed by Disease Activity Score 28 (DAS28), was 4.45. Basal cortisol and the percentage of cortisol reduction after the very low dose intravenous dexamethasone suppression test, as well as NF-κB, IKK, c-Fos, and GRα mRNA expression, were similar among groups. We did not observe significant correlations between glucocorticoid in vivo sensitivity and DAS28. There was a positive correlation between DAS28 and NF-κB, IKK, and GRα, but not c-Fos. In the multivariate analysis, only NF-κB mRNA remained as an independent variable for predicting DAS28.
ISSN:1676-5680
1676-5680
DOI:10.4238/vol9-4gmr970