Comparing inactivation protocols of Yersinia organisms for identification with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

RATIONALE It is recommended that harmful Biosafety Level 3 (BSL‐3) bacteria be inactivated prior to identification by mass spectrometry, yet optimal effects of inactivation protocol have not been defined. METHODS Here, we compare trifluoroacetic acid inactivation (protocol A) with ethanol inactivati...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2012-03, Vol.26 (6), p.710-714
Main Authors: Couderc, Carine, Nappez, Claude, Drancourt, Michel
Format: Article
Language:English
Subjects:
Ethanol - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Trifluoroacetic Acid - metabolism
Yersinia - chemistry
Yersinia - isolation & purification
Yersinia - physiology
Yersinia Infections - microbiology
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Summary:RATIONALE It is recommended that harmful Biosafety Level 3 (BSL‐3) bacteria be inactivated prior to identification by mass spectrometry, yet optimal effects of inactivation protocol have not been defined. METHODS Here, we compare trifluoroacetic acid inactivation (protocol A) with ethanol inactivation (protocol B) of Yersinia organisms prior to identification by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS). RESULTS The total number of peaks detected was 10.5 ± 1.7 for protocol A and 15.7 ± 4.2 for protocol B (ρ
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.6152