The nucleotide polymorphisms within the Epstein–Barr virus C and Q promoters from nasopharyngeal carcinoma affect transcriptional activity in vitro

Epstein–Barr virus (EBV) is a ubiquitous human gamma herpesvirus that is associated with Burkitt’s lymphoma (BL), gastric carcinoma, nasopharyngeal carcinoma (NPC), and NK/T-cell lymphoma. Two viral promoters, Cp and Qp, are important for EBV latent infection. The latency Cp, which is used in primar...

Full description

Saved in:
Bibliographic Details
Published in:European archives of oto-rhino-laryngology 2012-03, Vol.269 (3), p.931-938
Main Authors: Wang, Feng-Wei, Wu, Xian-Rui, Liu, Wen-Ju, Liang, Ying-Jie, Huang, Yu-Fan, Liao, Yi-Ji, Shao, Chun-Kui, Zong, Yong-Sheng, Mai, Shi-Juan, Xie, Dan
Format: Article
Language:English
Subjects:
Carcinoma
DNA, Viral - genetics
Epstein-Barr Virus Nuclear Antigens - biosynthesis
Epstein-Barr Virus Nuclear Antigens - genetics
Gene Expression Regulation, Viral
Head and Neck Surgery
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - metabolism
Humans
Laryngology
Medicine
Medicine & Public Health
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms - genetics
Nasopharyngeal Neoplasms - pathology
Nasopharyngeal Neoplasms - virology
Neurosurgery
Otorhinolaryngology
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Promoter Regions, Genetic - genetics
Transcription, Genetic
Transcriptional Activation - genetics
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epstein–Barr virus (EBV) is a ubiquitous human gamma herpesvirus that is associated with Burkitt’s lymphoma (BL), gastric carcinoma, nasopharyngeal carcinoma (NPC), and NK/T-cell lymphoma. Two viral promoters, Cp and Qp, are important for EBV latent infection. The latency Cp, which is used in primary infection, drives expression of the full spectrum of EBV nuclear antigens. Qp is active in EBV-associated tumors and drives the latency I/II expression pattern. In this study, we determined nucleotides polymorphisms in the Cp and Qp promoter regions in peripheral blood mononuclear cells (PBMCs) from Cantonese healthy carriers and in biopsies of NPC, nasal NK/T lymphoma, BL, and gastric carcinoma. The sequence changes of −12G>T and +69 C>T in Cp and −197 G>A and +1 G>C in Qp were frequently identified in NPC. Transient transfection studies using luciferase gene reporters revealed a significant reduction (57.11%) in gene expression from the Cp +69T variant and increased expression (43.5%) from the Qp +1C variant compared to the prototype, suggesting that these sequence variations affect promoter activity. Our results indicate that the nucleotides polymorphisms in Cp and Qp occur frequently in NPC and might contribute to the oncogenesis of EBV.
ISSN:0937-4477
1434-4726
DOI:10.1007/s00405-011-1862-x