Early and late calcium waves during wound healing in corneal endothelial cells

Immediately after wounding, bovine corneal endothelial cells develop a fast calcium wave that propagates from the wound border to the rest of the monolayer and extinguishes in approximately 5 minutes. One hour after wounding, a late, slow calcium wave (SCW) develops concomitantly to the depolarizati...

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Bibliographic Details
Published in:Wound repair and regeneration 2012-01, Vol.20 (1), p.28-37
Main Authors: Chifflet, Silvia, Justet, Cristian, Hernández, Julio A., Nin, Verónica, Escande, Carlos, Benech, Juan C.
Format: Article
Language:English
Subjects:
Animals
Calcium Channels, L-Type - pharmacology
Calcium Signaling
Cattle
Cells, Cultured
Cornea - cytology
Cornea - pathology
Endothelial Cells - pathology
Endothelial Growth Factors
Wound Healing
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Summary:Immediately after wounding, bovine corneal endothelial cells develop a fast calcium wave that propagates from the wound border to the rest of the monolayer and extinguishes in approximately 5 minutes. One hour after wounding, a late, slow calcium wave (SCW) develops concomitantly to the depolarization of the plasma membrane potential of the border cells. The incorporation of inhibitors of the epithelial sodium channel and of the sodium‐calcium exchanger produces inhibition of the membrane depolarization and the SCW, and diminishes the rate of wound healing. The L‐type calcium channel blocker nimodipine does not have any effect on the SCW. The reversible inhibition of the fast calcium wave does not affect the SCW and only slightly decreases the velocity of healing. Our results suggest that the SCW is at least partially produced by the coupling of the epithelial sodium channel and the sodium‐calcium exchanger functioning in reverse mode. They also suggest that the SCW may play a role in the overall healing process.
ISSN:1067-1927
1524-475X
DOI:10.1111/j.1524-475X.2011.00749.x