New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis

Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. sui...

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Bibliographic Details
Published in:Veterinary microbiology 2011-12, Vol.154 (1), p.152-155
Main Authors: López-Goñi, Ignacio, García-Yoldi, David, Marín, Clara M., de Miguel, María J., Barquero-Calvo, Elías, Guzmán-Verri, Caterina, Albert, David, Garin-Bastuji, Bruno
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Bruce-ladder
Brucella canis
Brucella canis - classification
Brucella canis - genetics
Brucella suis
Brucella suis - classification
Brucella suis - genetics
Brucellosis - diagnosis
Canis
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Microbiology
Miscellaneous
Multiplex Polymerase Chain Reaction - methods
PCR
Sequence Analysis, DNA
Species Specificity
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Summary:Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows the correct discrimination of all known Brucella species is also described.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2011.06.035