Loading…
Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum
Hot aqueous extraction of Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH 2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25 °C, gave nonreducing o...
Saved in:
Published in: | Carbohydrate polymers 2011-05, Vol.85 (2), p.349-355 |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213 |
---|---|
cites | cdi_FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213 |
container_end_page | 355 |
container_issue | 2 |
container_start_page | 349 |
container_title | Carbohydrate polymers |
container_volume | 85 |
creator | Barreto-Bergter, E. Sassaki, G.L. Souza, L.M. Rollin, R.R. Wagner, R. Bittencourt, V.C.B. Lopes, L.C.L. Simas-Tosin, F.F. Noseda, M.D. Gorin, P.A.J. |
description | Hot aqueous extraction of
Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH
2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25
°C, gave nonreducing oligosaccharide epitopes (OLIGO-Sa) from
O-linked protein and a polymer (HET-PR-Sa-de-O), which was then β-eliminated at 100
°C to give polysaccharide (HET-Sa). The structure of each fraction (methylation, NMR, and ESI-MS analysis) differed from those of a peptidoglycan (PRM-Pb) from mycelium of related, opportunistic pathogen,
Pseudallescheria boydii. The predominant nonreducing oligosaccharide formed on β-elimination of PRM-Pb was hexasaccharide
3, whereas those (OLIGO-Sa) from HET-PR-Sa were tetra-
2 and mixed pentasaccharides. Trisaccharide
1 was also identified and is a conserved structure in both fungi. Structural differences confirmed that
S.
apiospermum is not an anamorph of a
P. boydii teleomorph. |
doi_str_mv | 10.1016/j.carbpol.2011.02.033 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_918043694</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0144861711001512</els_id><sourcerecordid>918043694</sourcerecordid><originalsourceid>FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213</originalsourceid><addsrcrecordid>eNqFkE2LFDEQhoMoOK77E4RcxFO3qaS_chIZ3F1hwYPuOaTTlZkM3Z2YpIX592aYwau5JAXPm6p6CPkArAYG3edTbXQcg59rzgBqxmsmxCuyg6GXFYimeU12DJqmGjro35J3KZ1YOR2wHcF9ifrjeYo6I8Xgsg-YqFvpYT4bH6LPWAob_ULzEakPwce8rS5lZ6jd1oOeadD56A-40p8GJ58K4baF6uDKG-OyLe_JG6vnhPe3-468PHz7tX-qnn88ft9_fa6M6CFXpu2aFseWtaIb5AjCouSa91JIbnWrucQJTT9NfBTYdbodQfd25NoOyIGDuCOfrv-WuX9vmLJaXDI4z3pFvyUlYWCN6GRTyPZKmuhTimhViG7R8ayAqYtVdVI3q-piVTGuitWS-3jroJPRs416NS79C_MGOJdw4b5cOSzr_nEYVTIO1-LHRTRZTd79p9NfcJuTSA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>918043694</pqid></control><display><type>article</type><title>Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum</title><source>ScienceDirect Journals</source><creator>Barreto-Bergter, E. ; Sassaki, G.L. ; Souza, L.M. ; Rollin, R.R. ; Wagner, R. ; Bittencourt, V.C.B. ; Lopes, L.C.L. ; Simas-Tosin, F.F. ; Noseda, M.D. ; Gorin, P.A.J.</creator><creatorcontrib>Barreto-Bergter, E. ; Sassaki, G.L. ; Souza, L.M. ; Rollin, R.R. ; Wagner, R. ; Bittencourt, V.C.B. ; Lopes, L.C.L. ; Simas-Tosin, F.F. ; Noseda, M.D. ; Gorin, P.A.J.</creatorcontrib><description>Hot aqueous extraction of
Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH
2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25
°C, gave nonreducing oligosaccharide epitopes (OLIGO-Sa) from
O-linked protein and a polymer (HET-PR-Sa-de-O), which was then β-eliminated at 100
°C to give polysaccharide (HET-Sa). The structure of each fraction (methylation, NMR, and ESI-MS analysis) differed from those of a peptidoglycan (PRM-Pb) from mycelium of related, opportunistic pathogen,
Pseudallescheria boydii. The predominant nonreducing oligosaccharide formed on β-elimination of PRM-Pb was hexasaccharide
3, whereas those (OLIGO-Sa) from HET-PR-Sa were tetra-
2 and mixed pentasaccharides. Trisaccharide
1 was also identified and is a conserved structure in both fungi. Structural differences confirmed that
S.
apiospermum is not an anamorph of a
P. boydii teleomorph.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2011.02.033</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Biological and medical sciences ; Carbohydrate epitopes ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Morphology, structure, chemical composition ; Mycology ; Natural polymers ; Physicochemistry of polymers ; Proteins ; Pseudallescheria boydii ; Scedosporium apiospermum</subject><ispartof>Carbohydrate polymers, 2011-05, Vol.85 (2), p.349-355</ispartof><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213</citedby><cites>FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24122913$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Barreto-Bergter, E.</creatorcontrib><creatorcontrib>Sassaki, G.L.</creatorcontrib><creatorcontrib>Souza, L.M.</creatorcontrib><creatorcontrib>Rollin, R.R.</creatorcontrib><creatorcontrib>Wagner, R.</creatorcontrib><creatorcontrib>Bittencourt, V.C.B.</creatorcontrib><creatorcontrib>Lopes, L.C.L.</creatorcontrib><creatorcontrib>Simas-Tosin, F.F.</creatorcontrib><creatorcontrib>Noseda, M.D.</creatorcontrib><creatorcontrib>Gorin, P.A.J.</creatorcontrib><title>Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum</title><title>Carbohydrate polymers</title><description>Hot aqueous extraction of
Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH
2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25
°C, gave nonreducing oligosaccharide epitopes (OLIGO-Sa) from
O-linked protein and a polymer (HET-PR-Sa-de-O), which was then β-eliminated at 100
°C to give polysaccharide (HET-Sa). The structure of each fraction (methylation, NMR, and ESI-MS analysis) differed from those of a peptidoglycan (PRM-Pb) from mycelium of related, opportunistic pathogen,
Pseudallescheria boydii. The predominant nonreducing oligosaccharide formed on β-elimination of PRM-Pb was hexasaccharide
3, whereas those (OLIGO-Sa) from HET-PR-Sa were tetra-
2 and mixed pentasaccharides. Trisaccharide
1 was also identified and is a conserved structure in both fungi. Structural differences confirmed that
S.
apiospermum is not an anamorph of a
P. boydii teleomorph.</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate epitopes</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition</subject><subject>Mycology</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>Proteins</subject><subject>Pseudallescheria boydii</subject><subject>Scedosporium apiospermum</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkE2LFDEQhoMoOK77E4RcxFO3qaS_chIZ3F1hwYPuOaTTlZkM3Z2YpIX592aYwau5JAXPm6p6CPkArAYG3edTbXQcg59rzgBqxmsmxCuyg6GXFYimeU12DJqmGjro35J3KZ1YOR2wHcF9ifrjeYo6I8Xgsg-YqFvpYT4bH6LPWAob_ULzEakPwce8rS5lZ6jd1oOeadD56A-40p8GJ58K4baF6uDKG-OyLe_JG6vnhPe3-468PHz7tX-qnn88ft9_fa6M6CFXpu2aFseWtaIb5AjCouSa91JIbnWrucQJTT9NfBTYdbodQfd25NoOyIGDuCOfrv-WuX9vmLJaXDI4z3pFvyUlYWCN6GRTyPZKmuhTimhViG7R8ayAqYtVdVI3q-piVTGuitWS-3jroJPRs416NS79C_MGOJdw4b5cOSzr_nEYVTIO1-LHRTRZTd79p9NfcJuTSA</recordid><startdate>20110506</startdate><enddate>20110506</enddate><creator>Barreto-Bergter, E.</creator><creator>Sassaki, G.L.</creator><creator>Souza, L.M.</creator><creator>Rollin, R.R.</creator><creator>Wagner, R.</creator><creator>Bittencourt, V.C.B.</creator><creator>Lopes, L.C.L.</creator><creator>Simas-Tosin, F.F.</creator><creator>Noseda, M.D.</creator><creator>Gorin, P.A.J.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>20110506</creationdate><title>Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum</title><author>Barreto-Bergter, E. ; Sassaki, G.L. ; Souza, L.M. ; Rollin, R.R. ; Wagner, R. ; Bittencourt, V.C.B. ; Lopes, L.C.L. ; Simas-Tosin, F.F. ; Noseda, M.D. ; Gorin, P.A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate epitopes</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition</topic><topic>Mycology</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><topic>Proteins</topic><topic>Pseudallescheria boydii</topic><topic>Scedosporium apiospermum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barreto-Bergter, E.</creatorcontrib><creatorcontrib>Sassaki, G.L.</creatorcontrib><creatorcontrib>Souza, L.M.</creatorcontrib><creatorcontrib>Rollin, R.R.</creatorcontrib><creatorcontrib>Wagner, R.</creatorcontrib><creatorcontrib>Bittencourt, V.C.B.</creatorcontrib><creatorcontrib>Lopes, L.C.L.</creatorcontrib><creatorcontrib>Simas-Tosin, F.F.</creatorcontrib><creatorcontrib>Noseda, M.D.</creatorcontrib><creatorcontrib>Gorin, P.A.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barreto-Bergter, E.</au><au>Sassaki, G.L.</au><au>Souza, L.M.</au><au>Rollin, R.R.</au><au>Wagner, R.</au><au>Bittencourt, V.C.B.</au><au>Lopes, L.C.L.</au><au>Simas-Tosin, F.F.</au><au>Noseda, M.D.</au><au>Gorin, P.A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum</atitle><jtitle>Carbohydrate polymers</jtitle><date>2011-05-06</date><risdate>2011</risdate><volume>85</volume><issue>2</issue><spage>349</spage><epage>355</epage><pages>349-355</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>Hot aqueous extraction of
Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH
2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25
°C, gave nonreducing oligosaccharide epitopes (OLIGO-Sa) from
O-linked protein and a polymer (HET-PR-Sa-de-O), which was then β-eliminated at 100
°C to give polysaccharide (HET-Sa). The structure of each fraction (methylation, NMR, and ESI-MS analysis) differed from those of a peptidoglycan (PRM-Pb) from mycelium of related, opportunistic pathogen,
Pseudallescheria boydii. The predominant nonreducing oligosaccharide formed on β-elimination of PRM-Pb was hexasaccharide
3, whereas those (OLIGO-Sa) from HET-PR-Sa were tetra-
2 and mixed pentasaccharides. Trisaccharide
1 was also identified and is a conserved structure in both fungi. Structural differences confirmed that
S.
apiospermum is not an anamorph of a
P. boydii teleomorph.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2011.02.033</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0144-8617 |
ispartof | Carbohydrate polymers, 2011-05, Vol.85 (2), p.349-355 |
issn | 0144-8617 1879-1344 |
language | eng |
recordid | cdi_proquest_miscellaneous_918043694 |
source | ScienceDirect Journals |
subjects | Applied sciences Biological and medical sciences Carbohydrate epitopes Exact sciences and technology Fundamental and applied biological sciences. Psychology Microbiology Morphology, structure, chemical composition Mycology Natural polymers Physicochemistry of polymers Proteins Pseudallescheria boydii Scedosporium apiospermum |
title | Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T03%3A34%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Carbohydrate%20epitopes%20in%20glycoprotein%20from%20the%20opportunistic%20fungal%20pathogen%20Scedosporium%20apiospermum&rft.jtitle=Carbohydrate%20polymers&rft.au=Barreto-Bergter,%20E.&rft.date=2011-05-06&rft.volume=85&rft.issue=2&rft.spage=349&rft.epage=355&rft.pages=349-355&rft.issn=0144-8617&rft.eissn=1879-1344&rft.coden=CAPOD8&rft_id=info:doi/10.1016/j.carbpol.2011.02.033&rft_dat=%3Cproquest_cross%3E918043694%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c371t-c5645eb5053689b13fe92a279392fa5a29edec7dd2b3e66a5b1a7fb2af8e21213%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=918043694&rft_id=info:pmid/&rfr_iscdi=true |