Carbohydrate epitopes in glycoprotein from the opportunistic fungal pathogen Scedosporium apiospermum

Hot aqueous extraction of Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH 2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25 °C, gave nonreducing o...

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Published in:Carbohydrate polymers 2011-05, Vol.85 (2), p.349-355
Main Authors: Barreto-Bergter, E., Sassaki, G.L., Souza, L.M., Rollin, R.R., Wagner, R., Bittencourt, V.C.B., Lopes, L.C.L., Simas-Tosin, F.F., Noseda, M.D., Gorin, P.A.J.
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Carbohydrate epitopes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Microbiology
Morphology, structure, chemical composition
Mycology
Natural polymers
Physicochemistry of polymers
Proteins
Pseudallescheria boydii
Scedosporium apiospermum
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Summary:Hot aqueous extraction of Scedosporium apiospermum mycelium provided glycoprotein (HET-PR-Sa) containing 37% protein and Rha, Rib, Ara, Man, Gal, Glc, and GlcNH 2 in a 26:14:17:22:10:5:6 molar ratio. HPSEC showed a mixture. HET-PR-Sa, on reductive, alkaline β-elimination at 25 °C, gave nonreducing oligosaccharide epitopes (OLIGO-Sa) from O-linked protein and a polymer (HET-PR-Sa-de-O), which was then β-eliminated at 100 °C to give polysaccharide (HET-Sa). The structure of each fraction (methylation, NMR, and ESI-MS analysis) differed from those of a peptidoglycan (PRM-Pb) from mycelium of related, opportunistic pathogen, Pseudallescheria boydii. The predominant nonreducing oligosaccharide formed on β-elimination of PRM-Pb was hexasaccharide 3, whereas those (OLIGO-Sa) from HET-PR-Sa were tetra- 2 and mixed pentasaccharides. Trisaccharide 1 was also identified and is a conserved structure in both fungi. Structural differences confirmed that S. apiospermum is not an anamorph of a P. boydii teleomorph.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2011.02.033