Isolation of human nasoseptal chondrogenic cells: A promise for cartilage engineering

In cartilaginous tissues, perichondrium cambium layer may be the source of new cartilage. Human nasal septal perichondrium is considered to be a homogeneous structure in which some authors do not recognize the perichondrium internal zone or the cambium layer as a layer distinct from adjacent cartila...

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Published in:Stem cell research 2012-03, Vol.8 (2), p.292-299
Main Authors: Amaral, Ronaldo J.F.C. do, Pedrosa, Carolina da S.G., Kochem, Michele C.L., Silva, Karina R. da, Aniceto, Marcelo, Claudio-da-Silva, Cesar, Borojevic, Radovan, Baptista, Leandra S.
Format: Article
Language:English
Subjects:
Adipogenesis
Adult
Cartilage - physiology
Cell Culture Techniques
Cell Lineage
Cell Separation - methods
Chondrocytes - cytology
Chondrocytes - ultrastructure
Chondrogenesis
Humans
Nasal Septum - cytology
Nasal Septum - ultrastructure
Osteogenesis
Tissue Engineering - methods
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Summary:In cartilaginous tissues, perichondrium cambium layer may be the source of new cartilage. Human nasal septal perichondrium is considered to be a homogeneous structure in which some authors do not recognize the perichondrium internal zone or the cambium layer as a layer distinct from adjacent cartilage surface. In the present study, we isolated a chondrogenic cell population from human nasal septal cartilage surface zone. Nasoseptal chondrogenic cells were positive for surface markers described for mesenchymal stem cells, with exception of CD146, a perivascular cell marker, which is consistent with their avascular niche in cartilage. Although only Sox-9 was constitutively expressed, they also revealed osteogenic and chondrogenic, but not adipogenic, potentials in vitro, suggesting a more restricted lineage potential compared to mesenchymal stem cells. Interestingly, even in absence of chondrogenic growth factors in the pellet culture system, nasoseptal chondrogenic cells had a capacity to synthesize sulfated glycosaminoglycans, large amounts of collagen type II and to a lesser extent collagen type I. The spontaneous chondrogenic potential of this population of cells indicates that they may be a possible source for cartilage tissue engineering. Besides, the pellet culture system using nasoseptal chondrogenic cells may also be a model for studies of chondrogenesis. ► We isolated and characterized a cell population from human nasal septal cartilage. ► Cells dwell on cartilage surface, which correspond to perichondrium cambium layer. ► Cells showed a more restricted lineage potential compared to mesenchymal stem cells. ► Cells pellets extracellular matrix is rich in collagen type II and glycosaminoglycans. ► No growth factors are needed to promote cell chondrogenic differentiation.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2011.09.006