Gold nanoparticles generated and stabilized by water soluble curcumin–polymer conjugate: Blood compatibility evaluation and targeted drug delivery onto cancer cells

[Display omitted] ► Curcumin conjugated AuNPs were synthesized using hyaluronic acid-curcumin conjugates. ► HA–Cur@AuNPs were further HA–Cur@AuNPs modified with folate conjugated PEG to improve the cancer cell targeting. ► PF.HA–Cur@AuNPs exhibited better dose dependent cytotoxicity toward different...

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Bibliographic Details
Published in:Journal of colloid and interface science 2012-02, Vol.368 (1), p.144-151
Main Authors: Manju, S., Sreenivasan, K.
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
bioavailability
Biocompatibility
Biocompatible Materials
Blood
Blood Coagulation - drug effects
Cancer
Cell Proliferation - drug effects
Chemistry
Colloidal state and disperse state
Confocal
confocal microscopy
curcumin
Curcumin - pharmacology
cytotoxicity
Dose-Response Relationship, Drug
Drug Delivery Systems
Drug Screening Assays, Antitumor
Exact sciences and technology
Flow cytometry
folic acid
Folic acid conjugated polyethylene glycol
General and physical chemistry
Gold
Gold - chemistry
Gold nanoparticle
HeLa Cells
Hemocompatibility
Humans
Hyaluronic acid
Hyaluronic Acid - chemistry
Magnetics
Membranes, Artificial
Metal Nanoparticles
nanocarriers
nanogold
Nanoparticles
Nanostructure
neoplasm cells
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Solubility
Structure-Activity Relationship
Surface Properties
Tumor Cells, Cultured
Water - chemistry
Water soluble curcumin
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Summary:[Display omitted] ► Curcumin conjugated AuNPs were synthesized using hyaluronic acid-curcumin conjugates. ► HA–Cur@AuNPs were further HA–Cur@AuNPs modified with folate conjugated PEG to improve the cancer cell targeting. ► PF.HA–Cur@AuNPs exhibited better dose dependent cytotoxicity toward different cancer cell lines. ► PF.HA–Cur@AuNPs demonstrated enhanced targeting efficacy of 95.4%. Curcumin (Cur) shows low anticancer activity in vivo due to its reduced systemic bioavailability stemmed from its poor aqueous solubility and instability. Suitably functionalized nanocarriers designed to empty the drug specifically at tumor sites can potentially enhance the antitumor activity of Cur. We devised a simple method for the fabrication of water soluble Cur conjugated gold nanoparticles to target various cancer cell lines. Cur was conjugated to hyaluronic acid (HA) to get a water soluble conjugate (HA–Cur). We generated gold nanoparticles (AuNPs) by reducing chloroauric acid using HA–Cur, which played the dual role of a reducing and stabilizing agent and subsequently anchored folate conjugated PEG. These entities were probed using different analytical techniques, assayed the blood compatibility and cytotoxicity. Their interaction with cancer cell lines (HeLa cells, glyoma cells and Caco 2 cells) was followed by flow cytometry and confocal microscopy. Blood–materials interactions studies showed that the nanoparticles are highly hemocompatible. Flow cytometry and confocal microscopy results showed significant cellular uptake and internalization of the particles by cells. HA–Cur@AuNPs exhibited more cytotoxicity comparing to free Cur. The strategy, we adopted here, resulted the formation blood compatible Cur conjugated AuNPs with enhanced targeting and improved efficacy.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2011.11.024