Evaluating the use of optical coherence tomography for the detection of epithelial cancers in vitro

Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolv...

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Published in:Journal of biomedical optics 2011-11, Vol.16 (11), p.116015-116015
Main Authors: Smith, Louise E, Hearnden, Vanessa, Lu, Zenghai, Smallwood, Rod, Hunter, Keith D, Matcher, Stephen J, Thornhill, Martin H, Murdoch, Craig, MacNeil, Sheila
Format: Article
Language:English
Subjects:
Carcinoma, Squamous Cell - chemistry
Cell Line, Tumor
Cells, Cultured
Fingers
Histocytochemistry
Humans
Melanoma - chemistry
Models, Biological
Mouth Mucosa - chemistry
Mouth Neoplasms - chemistry
Skin - chemistry
Skin Neoplasms - chemistry
Tomography, Optical Coherence - methods
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cited_by cdi_FETCH-LOGICAL-c249t-9973e2f5cb8761c27c8fc33f42896f4f0edc6c38ced9df2ed66c460e31bc90ed3
cites cdi_FETCH-LOGICAL-c249t-9973e2f5cb8761c27c8fc33f42896f4f0edc6c38ced9df2ed66c460e31bc90ed3
container_end_page 116015
container_issue 11
container_start_page 116015
container_title Journal of biomedical optics
container_volume 16
creator Smith, Louise E
Hearnden, Vanessa
Lu, Zenghai
Smallwood, Rod
Hunter, Keith D
Matcher, Stephen J
Thornhill, Martin H
Murdoch, Craig
MacNeil, Sheila
description Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial structure could greatly improve early diagnosis and long-term monitoring in patients. Here, we have used tissue-engineered (TE) models of normal skin and oral mucosa to generate models of melanoma and oral cancer. We have used these to determine the ability of OCT to image epithelial differences in vitro. We report that while in vivo OCT gives reasonable depth information for both skin and oral mucosa, in vitro the information provided is less detailed but still useful. OCT can provide reassurance on the development of TE models of skin and oral mucosa as they develop in vitro. OCT was able to detect the gross alteration in the epithelium of skin and mucosal models generated with malignant cell lines but was less able to detect alteration in the epithelium of TE models that mimicked oral dysplasia or, in models where tumor cells had penetrated into the dermis.
doi_str_mv 10.1117/1.3652708
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subjects Carcinoma, Squamous Cell - chemistry
Cell Line, Tumor
Cells, Cultured
Fingers
Histocytochemistry
Humans
Melanoma - chemistry
Models, Biological
Mouth Mucosa - chemistry
Mouth Neoplasms - chemistry
Skin - chemistry
Skin Neoplasms - chemistry
Tomography, Optical Coherence - methods
title Evaluating the use of optical coherence tomography for the detection of epithelial cancers in vitro
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