Evaluating the use of optical coherence tomography for the detection of epithelial cancers in vitro

Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolv...

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Bibliographic Details
Published in:Journal of biomedical optics 2011-11, Vol.16 (11), p.116015-116015
Main Authors: Smith, Louise E, Hearnden, Vanessa, Lu, Zenghai, Smallwood, Rod, Hunter, Keith D, Matcher, Stephen J, Thornhill, Martin H, Murdoch, Craig, MacNeil, Sheila
Format: Article
Language:English
Subjects:
Carcinoma, Squamous Cell - chemistry
Cell Line, Tumor
Cells, Cultured
Fingers
Histocytochemistry
Humans
Melanoma - chemistry
Models, Biological
Mouth Mucosa - chemistry
Mouth Neoplasms - chemistry
Skin - chemistry
Skin Neoplasms - chemistry
Tomography, Optical Coherence - methods
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Summary:Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial structure could greatly improve early diagnosis and long-term monitoring in patients. Here, we have used tissue-engineered (TE) models of normal skin and oral mucosa to generate models of melanoma and oral cancer. We have used these to determine the ability of OCT to image epithelial differences in vitro. We report that while in vivo OCT gives reasonable depth information for both skin and oral mucosa, in vitro the information provided is less detailed but still useful. OCT can provide reassurance on the development of TE models of skin and oral mucosa as they develop in vitro. OCT was able to detect the gross alteration in the epithelium of skin and mucosal models generated with malignant cell lines but was less able to detect alteration in the epithelium of TE models that mimicked oral dysplasia or, in models where tumor cells had penetrated into the dermis.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.3652708