Glycoproteins are species-specific markers and major IgE reactants in grass pollens

Summary Grass pollen allergic patients are concomitantly exposed and sensitized to pollens from multiple Pooideae (i.e. common grass) species. As such, they are currently desensitized by allergen‐specific immunotherapy using extracts made from mixes of pollens from Anthoxanthum odoratum, Dactylis gl...

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Published in:Plant biotechnology journal 2012-02, Vol.10 (2), p.184-194
Main Authors: Manduzio, Hélène, Fitchette, Anne-Catherine, Hrabina, Maud, Chabre, Henri, Batard, Thierry, Nony, Emmanuel, Faye, Loïc, Moingeon, Philippe, Gomord, Véronique
Format: Article
Language:English
Subjects:
Allergens - immunology
allergy
Biological and medical sciences
Biomarkers
Biotechnology
Cross Reactions
cross-reactive carbohydrate determinant
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
glycan-specific IgE
glycoepitope
Glycoproteins - immunology
glycoproteome
grass pollen
Immunoglobulin E - immunology
Poaceae - immunology
Pollen - immunology
Species Specificity
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Summary:Summary Grass pollen allergic patients are concomitantly exposed and sensitized to pollens from multiple Pooideae (i.e. common grass) species. As such, they are currently desensitized by allergen‐specific immunotherapy using extracts made from mixes of pollens from Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Phleum pratense and Poa pratensis. Herein, we demonstrate that species‐specific glycoprotein patterns are documented by 1D and 2D electrophoresis and Western blotting analysis, which can be used as an identity test for such pollens. Most allergens are glycoproteins bearing complex N‐glycans encompassing β1,2 xylose and α1,3 fucose glycoepitopes. Glycoepitope destruction using periodate oxidation has no impact on seric IgE reactivity in 75% atopic patients (n = 24). The latter have thus no significant IgE responses to carbohydrate‐containing epitopes. In contrast, periodate treatment strongly impairs IgE recognition of glycoallergens in 25% of patients tested, demonstrating the presence of carbohydrate‐specific IgE in those patients. While the clinical impact of carbohydrate‐specific IgE is still a matter of controversy, the presence of these IgE in the serum of many allergic patients illustrates the need for cross‐reacting carbohydrate epitope‐free recombinant allergens to develop relevant diagnostic tests. These data also support the pertinence of mixing multiple grass pollens to desensitize atopic patients, with the aim to broaden the repertoire of glycoepitopes in the vaccine, thus mimicking natural exposure conditions.
ISSN:1467-7644
1467-7652
DOI:10.1111/j.1467-7652.2011.00654.x