Different patterns of gene expression in rice varieties undergoing a resistant or susceptible interaction with the bacterial leaf streak pathogen

The rice line carrying the nonhost gene Rxo1, which was cloned from maize, exhibits a rapid hypersensitive response (HR) to Xanthomonas oryzae pv. oryzicola (Xoc). In this study, a microarray experiment was carried out to analyze the genome-wide gene expression responses to Xoc at the early stage in...

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Bibliographic Details
Published in:African journal of biotechnology 2011-10, Vol.10 (65), p.14419-14438
Main Authors: Mei Rong, Xu, Casiana, M Vera Cruz, Bin Ying, Fu, Ling Hua, Zhu, Yong Li, Zhou, Zhi Kang, Li
Format: Article
Language:English
Subjects:
Bacteria
Oryza sativa
Xanthomonas oryzae
Zea mays
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Summary:The rice line carrying the nonhost gene Rxo1, which was cloned from maize, exhibits a rapid hypersensitive response (HR) to Xanthomonas oryzae pv. oryzicola (Xoc). In this study, a microarray experiment was carried out to analyze the genome-wide gene expression responses to Xoc at the early stage in the rice transgenic line and its wild type. 175 and 379 differentially regulated genes (DRGs) were detected in 9804-Rxo1 and 9804 at 36 h post-inoculation (hpi), respectively. The patterns of DRGs induced by Xoc in the transgenic rice line and its wild type were distinctly different: 92.00% of the DRGs were up-regulated in inoculated 9804-Rxo1 and 48.22% of the DRGs were sorted as defense-related genes. In contrast, 40.69% of the DRGs were up-regulated in inoculated 9804 and 40.00% were sorted as injury-induced genes. Some genes specifically up-regulated in infected 9804-Rxo1 were defense-related, including the genes encoding pathogenesis-related protein, terpene synthase family, transcription factors (TFs) AP2 domain containing protein, myb-like deoxyribonucleic acid (DNA)-binding domain containing protein, and C sub(2)H sub(2)-type zinc-finger transcription factors. Six peroxidase (POD) genes were significantly up-regulated and POD activity consistently rose in the inoculated 9804-Rxo1, whereas seven POD genes were down-regulated in its wild type, indicating that POD may also play a role in the complex process of resistant response to Xoc in the transgenic line. Instead of focusing only on the R-gene-mediated defense, this study also discusses the susceptible response in the wild types to virulent pathogen infection in transcription level.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB11.1317