Adipocyte-secreted factors increase osteoblast proliferation and the OPG/RANKL ratio to influence osteoclast formation

► It is unclear whether fat mass increases bone density only by the higher weight. ► We investigate the role of adipocytes as an endocrine tissue in this context. ► Adipocyte-secreted factors include bFGF. ► These factors increase preosteoblast proliferation and OPG/RANKL ratio. ► These findings may...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2012-02, Vol.349 (2), p.180-188
Main Authors: Kühn, Markus C., Willenberg, Holger S., Schott, Matthias, Papewalis, Claudia, Stumpf, Ulla, Flohé, Sascha, Scherbaum, Werner A., Schinner, Sven
Format: Article
Language:English
Subjects:
Aadipocytes
Adipocytes - metabolism
Adipocytes - secretion
Animals
bFGF
Biocompatibility
Biomedical materials
Bones
Cell Differentiation
Cell Proliferation
Cells, Cultured
Culture Media, Conditioned
Density
Enzyme-Linked Immunosorbent Assay
Human
Humans
Inhibitors
Kinases
Mice
Obesity
OPG/RANKL ratio
Osteoblasts
Osteoblasts - cytology
Osteoblasts - metabolism
Osteoclasts - cytology
Osteoclasts - metabolism
Osteogenesis - physiology
Osteoporosis
Osteoprotegerin - genetics
Osteoprotegerin - metabolism
Phosphatidylinositol 3-Kinases - antagonists & inhibitors
Phosphatidylinositol 3-Kinases - genetics
Phosphatidylinositol 3-Kinases - metabolism
Protein Kinase Inhibitors - pharmacology
Proteins
RANK Ligand - genetics
RANK Ligand - metabolism
Real-Time Polymerase Chain Reaction
Receptor, Fibroblast Growth Factor, Type 1 - genetics
Receptor, Fibroblast Growth Factor, Type 1 - metabolism
RNA, Messenger - metabolism
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Summary:► It is unclear whether fat mass increases bone density only by the higher weight. ► We investigate the role of adipocytes as an endocrine tissue in this context. ► Adipocyte-secreted factors include bFGF. ► These factors increase preosteoblast proliferation and OPG/RANKL ratio. ► These findings may represent an endocrine effect of adipocytes on osteoblasts. Several studies have reported a positive relationship of the body fat mass and bone density. However, it is not clear whether adipocyte-derived signaling molecules directly act on osteoblasts or osteoclasts. Therefore, we investigated the effect of fat cell-secreted factors on the proliferation and differentiation of preosteoblasts and the molecular mechanisms involved. This stimulation led to an increased proliferation of MC3T3-E1 and primary preosteoblastic cells (2.8-fold and 1.5-fold, respectively; p
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2011.10.018