Flow injection chemiluminescence determination of vitamin B12 using on-line UV-persulfate photooxidation and charge coupled device detection

ABSTRACT A sensitive chemiluminescence method for vitamin B12 using a charge‐coupled device (CCD) photodetector combined with on‐line UV‐persulfate oxidation in a simple continuous flow system has been developed. The principle for the determination of vitamin B12 is based on the enhancive effect of...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2011-11, Vol.26 (6), p.536-542
Main Authors: Murillo Pulgarín, José A., García Bermejo, Luisa F., Nieves Sánchez García, M.
Format: Article
Language:English
Subjects:
charge coupled device
chemiluminescence
flow injection
Flow Injection Analysis - methods
Luminescence
Oxidation-Reduction
Photochemistry
Ultraviolet Rays
UV-persulfate oxidation
Vitamin B 12 - analysis
vitamin B12
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Summary:ABSTRACT A sensitive chemiluminescence method for vitamin B12 using a charge‐coupled device (CCD) photodetector combined with on‐line UV‐persulfate oxidation in a simple continuous flow system has been developed. The principle for the determination of vitamin B12 is based on the enhancive effect of cobalt (II) on the chemiluminescence reaction between luminol and percarbonate in alkaline medium. In addition, percarbonate has been investigated and proposed as a powerful source of hydrogen peroxide as oxidant agent in this chemiluminescence reaction. The digestion of vitamin B12 to release the cobalt (II) is reached by UV irradiation treatment in a persulfate medium. The CCD detector, directly connected to the flow cell, is used with the continuous flow manifold to obtain the full spectral characteristics of cobalt (II) catalyzed luminol–percarbonate reaction. The vitamin B12 oxidation process and chemical conditions for the chemiluminescence reaction were investigated and optimized. The increment of the emission intensity was proportional to the concentration of vitamin B12, giving a second‐order calibration graph over the cobalt (II) concentration range from 10 to 5000 μg L−1 (r2 = 0.9985) with a detection limit of 9.3 μg L−1. The proposed method was applied to the determination of vitamin B12 in different kinds of pharmaceuticals. Copyright © 2011 John Wiley & Sons, Ltd.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.1266