Primary human epithelial cell culture system for studying interactions between female upper genital tract and sexually transmitted viruses, HSV-2 and HIV-1

Evidence from clinical and epidemiological studies indicates that women are disproportionately susceptible to sexually transmitted viral infections. To understand the underlying biological basis for this increased susceptibility, more studies are needed to examine the acute events in the female repr...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2011-10, Vol.55 (2), p.114-121
Main Authors: Kaushic, Charu, Nazli, Aisha, Ferreira, Victor H., Kafka, Jessica K.
Format: Article
Language:English
Subjects:
Adult
Cell culture
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Cell Shape
Cells, Cultured
Differentiation
Disease transmission
Electric Impedance
Epithelial cells
Epithelial Cells - immunology
Epithelial Cells - metabolism
Epithelial Cells - virology
Ex-vivo primary culture
Female
Female reproductive tract
Genital tract
Herpes Genitalis - virology
Herpesvirus 2, Human - physiology
HIV Infections - virology
HIV-1
HIV-1 - physiology
Host-Pathogen Interactions
Human immunodeficiency virus 1
Humans
Hysterectomy
Infection
Microenvironments
Middle Aged
Polarized epithelial cells
Primary Cell Culture
Reproductive system
Sexually transmitted infection
Tight Junctions - metabolism
Trans-epithelial resistance
Uterus - pathology
Uterus - surgery
Uterus - virology
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Summary:Evidence from clinical and epidemiological studies indicates that women are disproportionately susceptible to sexually transmitted viral infections. To understand the underlying biological basis for this increased susceptibility, more studies are needed to examine the acute events in the female reproductive tract following exposure to viruses during sexual transmission. The epithelial lining of the female reproductive tract is the primary barrier that sexually transmitted viruses, such as HIV-1 and HSV-2 need to infect or traverse, in order to initiate and establish productive infection. We have established an ex-vivo primary culture system to grow genital epithelial cells from upper reproductive tract tissues of women. Using these cultures, we have extensively examined the interactions between epithelial cells of the female genital tract and HSV-2 and HIV-1. In this review, we describe in detail the experimental protocol to grow these cultures, monitor their differentiation and inoculate with HSV-2 and HIV-1. Prospective use of these cultures to re-create the microenvironment in the reproductive tract is discussed.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2011.09.022