Enrichment of functionally distinct mouse hematopoietic progenitor cell populations using CD62L

The details of the bifurcation of the lymphoid and myeloid lineages following commitment by multipotent progenitor cells (MPP) remain a topic of controversy. We report that the surface glycoprotein CD62L can be characterized as a novel marker of this and other stages of early hematopoietic different...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2011-11, Vol.187 (10), p.5203-5210
Main Authors: Cho, Scott, Spangrude, Gerald J
Format: Article
Language:English
Subjects:
Animals
Antigens, Ly - biosynthesis
Antigens, Ly - blood
Biomarkers - blood
Cell Differentiation - immunology
Cell Lineage - immunology
fms-Like Tyrosine Kinase 3 - biosynthesis
Hematopoietic Stem Cell Transplantation - methods
L-Selectin - biosynthesis
L-Selectin - immunology
Membrane Proteins - biosynthesis
Membrane Proteins - blood
Mice
Mice, Inbred C57BL
Mice, Transgenic
Multipotent Stem Cells - immunology
Multipotent Stem Cells - metabolism
Proto-Oncogene Proteins c-kit - biosynthesis
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Summary:The details of the bifurcation of the lymphoid and myeloid lineages following commitment by multipotent progenitor cells (MPP) remain a topic of controversy. We report that the surface glycoprotein CD62L can be characterized as a novel marker of this and other stages of early hematopoietic differentiation. Cell isolation and transplant studies demonstrated CD62L(neg/low) long-term hematopoietic stem cells and CD62L(high) MPP within the traditionally defined c-kit(pos)Lin(neg/low)Sca-1(pos) stem/progenitor cell population. Within the MPP population, previously defined as c-kit(pos)Lin(neg/low)Sca-1(pos)-Thy-1.1(neg)Flt3(pos), Sca-1 and CD62L resolved four populations and segregated Sca-1(high)CD62L(neg/low) MPP from Sca-1(high)CD62L(high) leukocyte-biased progenitors. Using a novel transplantation method that allows tracking of erythroid and platelet engraftment as an alternative to the classical method of in vitro colony formation, we characterized Sca-1(high)CD62L(neg/low) cells as MPP, based on transient engraftment of these lineages. These data establish CD62L as a useful tool in the study of early hematopoiesis and emphasize the power of trilineage-engraftment studies in establishing the lineage potential of MPP subsets.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.1102119