Relative in vitro effectiveness of several gonadal steroids on oocyte maturation in freshwater teleost Barilius vagra

The potency of various estrogens, androgens and progestogens at different concentrations and incubation periods on inducing final oocyte maturation in vitro was investigated. At the highest concentration (1000 ng/ml), all of the C sub(21) steroids tested, progesterone (P sub(4)), 17 alpha -hydroxypr...

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Bibliographic Details
Published in:	African journal of biotechnology 2011-09, Vol.10 (55), p.11772-11777
Main Authors:	Zuberi, A, Naeem, M, Jalali, S
Format:	Article
Language:	eng
Subjects:	Barilius vagra Freshwater Teleostei
Online Access:	Get full text
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Summary:	The potency of various estrogens, androgens and progestogens at different concentrations and incubation periods on inducing final oocyte maturation in vitro was investigated. At the highest concentration (1000 ng/ml), all of the C sub(21) steroids tested, progesterone (P sub(4)), 17 alpha -hydroxyprogesteron (17-OHP), 17 alpha , 20 beta -dihydroxy-4-pregnen-3-one (17, 20 beta P), as well as C sub(19) steroids, testosterone (T) and androsteindione (AD) were able to induce germinal vesicle breakdown (GVBD), whereas C sub(18) steroids, estrogens (E sub(1) and E sub(2)) were found totally ineffective in inducing oocyte maturation at all the concentrations and exposure time. The relative potency of steroids were progestogens > androgens > estrogens. Among the steroids tested, 17, 20 beta P appeared as the most potent oocyte maturation inducing steroid, causing 46% GVBD at the lower concentration of 10 ng/ml at 72 h exposure. The next most potent steroid was 17-OHP, inducing 25% GVBD at 72 h. The relative response among progestogens were 17, 20 beta P > 17-OHP > P sub(4). These data suggest that 17, 20 beta P is the major maturation-inducing steroid (MIS) in Barilius vagra and both the duration of exposure and the hormonal concentration had a positive influence on the percentage of germinal vesicle breakdown.
ISSN:	1684-5315 1684-5315