A distinct freshwater-adapted subgroup of ANME-1 dominates active archaeal communities in terrestrial subsurfaces in Japan

Summary Anaerobic methane‐oxidizing archaea (ANME) are known to play an important role in methane flux, especially in marine sediments. The 16S rRNA genes of ANME have been detected in terrestrial freshwater subsurfaces. However, it is unclear whether ANME are actively involved in methane oxidation...

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Published in:Environmental microbiology 2011-12, Vol.13 (12), p.3206-3218
Main Authors: Takeuchi, Mio, Yoshioka, Hideyoshi, Seo, Yuna, Tanabe, Susumu, Tamaki, Hideyuki, Kamagata, Yoichi, Takahashi, Hiroshi A., Igari, Shunichiro, Mayumi, Daisuke, Sakata, Susumu
Format: Article
Language:English
Subjects:
Adaptation, Biological
Anaerobiosis
Archaea
Archaea - classification
Archaea - genetics
Archaea - metabolism
Bacteria - classification
Bacteria - genetics
DNA, Archaeal - genetics
Ecosystem
Fresh Water - chemistry
Fresh Water - microbiology
Gene Library
Geologic Sediments - chemistry
Geologic Sediments - microbiology
Japan
Methane - metabolism
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
RNA, Ribosomal, 16S - genetics
Salinity
Water Microbiology
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Summary:Summary Anaerobic methane‐oxidizing archaea (ANME) are known to play an important role in methane flux, especially in marine sediments. The 16S rRNA genes of ANME have been detected in terrestrial freshwater subsurfaces. However, it is unclear whether ANME are actively involved in methane oxidation in these environments. To address this issue, Holocene sediments in the subsurface of the Kanto Plain in Japan were collected for biogeochemical and molecular analysis. The potential activity of the anaerobic oxidation of methane (AOM) (0.38–3.54 nmol cm−3 day−1) was detected in sediment slurry incubation experiments with a 13CH4 tracer. Higher AOM activity was observed in low‐salinity treatment compared with high‐salinity condition (20‰), which supports the adaptation of ANME in freshwater habitats. The 16S rRNA sequence analysis clearly revealed the presence of a distinct subgroup of ANME‐1, designated ANME‐1a‐FW. Phylogenetic analysis of the mcrA genes also implied the presence of the distinct subgroup in ANME‐1. ANME‐1a‐FW was found to be the most dominant active group in the archaeal communities on the basis of 16S rRNA analysis (75.0–93.8% of total archaeal 16S rRNA clones). Sulfate‐reducing bacteria previously known as the syntrophic bacterial partners of ANME‐1 was not detected. Our results showed that ANME‐1a‐FW is adapted to freshwater habitats and is responsible for AOM in terrestrial freshwater subsurface environments.
ISSN:1462-2912
1462-2920
DOI:10.1111/j.1462-2920.2011.02517.x