Proteome and cytokine serum profiling to diagnose a mycosis fungoides

Purpose: The aim of this study was to estimate a possibility of mycosis fungoides (MF) diagnostics based on protein profiling in blood serum. Experimental design: We obtained and analysed samples of blood serum from 23 patients with MF, and 29 psoriasis patients and 22 healthy donors as controls. Pr...

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Bibliographic Details
Published in:Proteomics. Clinical applications 2011-08, Vol.5 (7-8), p.432-439
Main Authors: Moshkovskii, Sergei A., Sokolova, Elizaveta E., Brattseva, Ekaterina V., Karpova, Maria A., Pyatnitskiy, Mikhail A., Kubanova, Anna A., Archakov, Alexander I.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarker
biomarkers
Biomarkers - blood
Blood
Case-Control Studies
Chemokine CXCL10 - blood
Chemokines
Cutaneous T-cell lymphoma
CXCL10
CXCL10 protein
Cytokines
Data processing
Diagnosis, Differential
Differentiation
Female
Fungal infections
Granulocyte colony-stimulating factor
Granulocyte Colony-Stimulating Factor - blood
Humans
Immunoassay - methods
Immunoassays
Interleukin 1 receptor antagonist
Interleukin 1 Receptor Antagonist Protein - blood
Interleukin 4
Interleukin-4 - blood
IP-10 protein
Male
Middle Aged
Mycosis fungoides
Mycosis Fungoides - blood
Mycosis Fungoides - diagnosis
Protein Array Analysis
Proteome - analysis
proteomics
Psoriasis
Psoriasis - blood
Psoriasis - diagnosis
Skin
Skin Neoplasms - blood
Skin Neoplasms - diagnosis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Statistics
Therapeutic applications
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Summary:Purpose: The aim of this study was to estimate a possibility of mycosis fungoides (MF) diagnostics based on protein profiling in blood serum. Experimental design: We obtained and analysed samples of blood serum from 23 patients with MF, and 29 psoriasis patients and 22 healthy donors as controls. Protein profiling was carried out using SELDI TOF MS SELDI‐TOF and also profiling of 27 cytokines with multiplex immunoassay technology was implemented. Results: MS data analysis of sera did not give satisfactory statistical discrimination between the groups. Antibody‐based cytokine profiling revealed a number of cytokines with a change in their concentrations in both MF and psoriasis (IL‐1Ra, IL‐4, G‐CSF). The C‐X‐C motif chemokine 10 (IP‐10, CXCL10) cytokine had a significantly increased concentration (p
ISSN:1862-8346
1862-8354
DOI:10.1002/prca.201000165