Fetal muscle contains different CD34 super(+ cell subsets that distinctly differentiate into adipogenic, angiogenic and myogenic lineages)

We have previously demonstrated that CD34 super(+ cells isolated from fetal mouse muscles are an interesting source of myogenic progenitors. In the present work, we pinpoint the tissue location of these CD34) super(+) cells using cell surface and phenotype markers. In order to identify the myogenic...

Full description

Saved in:
Bibliographic Details
Published in:Stem cell research 2011-11, Vol.7 (3), p.230-243
Main Authors: Dupas, Tanaelle, Rouaud, Thierry, Rouger, Karl, Lieubeau, Blandine, Cario-Toumaniantz, Chrystelle, Fontaine-Perus, Josiane, Gardahaut, Marie-France, Auda-Boucher, Gwenola
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have previously demonstrated that CD34 super(+ cells isolated from fetal mouse muscles are an interesting source of myogenic progenitors. In the present work, we pinpoint the tissue location of these CD34) super(+) cells using cell surface and phenotype markers. In order to identify the myogenic population, we next purified different CD34 super(+ subsets, determined their expression of relevant lineage-related genes, and analyzed their differentiation capacities in vitro and in vivo. The CD34) super(+) population comprised a CD31 super(+/CD45) super(-) cell subset exhibiting endothelial characteristics and only capable of forming microvessels in vivo. The CD34 super(+/CD31) super(-)/CD45 super(-/Sca1) super(+) subpopulation, which is restricted to the muscle epimysium, displayed adipogenic differentiation both in vitro and in vivo. CD34 super(+/CD31) super(-)/CD45 super(-/Sca1) super(&)minu s; cells, localized in the muscle interstitium, transcribed myogenic genes, but did not display the characteristics of adult satellite cells. These cells were distinct from pericytes and fibroblasts. They were myogenic in vitro, and efficiently contributed to skeletal muscle regeneration in vivo, although their myogenic potential was lower than that of the unfractionated CD34 super(+ cell population. Our results indicate that angiogenic and adipogenic cells grafted with myogenic cells enhance their contribution to myogenic regeneration, highlighting the fundamental role of the microenvironment on the fate of transplanted cells.)
ISSN:1873-5061
DOI:10.1016/j.scr.2011.06.004