Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system

Several plasmids that contain genetically marked variants of the TnBP transposon isolated from Bordetella pertussis have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an E. coli K12 chromosome at a nonpermissive temperature (42°C) have...

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Bibliographic Details
Published in:Molecular genetics, microbiology and virology microbiology and virology, 2010-03, Vol.25 (1), p.8-13
Main Authors: Umyarov, A. M., Sinyashina, L. N., Amelina, I. P., Datsenko, K. A., Bol’shakova, T. N., Dobrynina, O. Yu, Karataev, G. I.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Bordetella pertussis
Chromosomes
Experimental Works
Life Sciences
Microbiology
Molecular Medicine
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Summary:Several plasmids that contain genetically marked variants of the TnBP transposon isolated from Bordetella pertussis have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an E. coli K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced and HPr- or enzyme I-deficient cointegrate formation between the plasmid and the K12 bacterial chromosome were detected. The introduction of the bvgAS operon of B. pertussis at the trans-position into the mutant E. coli K12 bacteria restores the frequency of the formation and resolution of RSBP-induced cointegrates.
ISSN:0891-4168
1934-841X
DOI:10.3103/S0891416810010027