Mycoplasma adaptation to stress conditions: Proteome shift in Mycoplasma hominis PG37 in response to starvation and low temperatures

Mycoplasma hominis is a widely spread mycoplasma (class Mollicutes), associated with socially important human diseases and contamination of cell cultures. Controlling infections caused by M. hominis depends on determining the molecular mechanisms responsible for the bacterium’s survival in unfavorab...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology (New York) 2011-10, Vol.45 (5), p.843-851
Main Authors: Chernov, V. M., Chernova, O. A., Baranova, N. B., Gorshkov, O. V., Medvedeva, E. S., Shaymardanova, G. F.
Format: Article
Language:English
Subjects:
Adaptations
Amino acids
Bacteria
Bacterial infections
Bacterial proteins
Biochemistry
Biomedical and Life Sciences
Carbohydrate metabolism
Carbohydrates
Cell culture
Cell cycle
Contamination
Environmental conditions
Human Genetics
Infection
Life Sciences
Molecular biology
Molecular modelling
Mollicutes
Mycoplasma hominis
Nucleotides
Protein turnover
Proteomics
Starvation
Stress
Stress response
Temperature effects
Transcription
Translation
virulence factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mycoplasma hominis is a widely spread mycoplasma (class Mollicutes), associated with socially important human diseases and contamination of cell cultures. Controlling infections caused by M. hominis depends on determining the molecular mechanisms responsible for the bacterium’s survival in unfavorable conditions. A proteome analysis employing 2-DIGE and MALDI TOF/TOF MS was applied to identify, for the first time, 53 proteins of M. hominis PG37 whose levels altered in bacteria cultivated in stress conditions (starvation and low temperature). According to the protein classification by functional category (clusters of orthologous groups of proteins, COG), 47 of the 53 mycoplasma proteins identified are involved in fundamental cellular and biochemical processes: translation (12; 22.64%), transcription (2; 3.77%), posttranslational modification (7; 13.20%), cell cycle control (2; 3.77%), energy production and conversion (6; 11.32%), carbohydrate transport and metabolism (3; 5.66%), amino acid transport and metabolism (8; 15.09%), nucleotide transport and metabolism (6; 11.32%), and inorganic ion transport and metabolism (1; 1.89%). For six proteins (11.32%), the function was not determined; 24 proteins (45.28%) were bacterial virulence factors. Those proteins of M. hominis PG37 whose expression is modulated in response to unfavorable environmental conditions are components of stress adaptation mechanisms in mycoplasma and potential targets for controlling infections caused by this bacterium.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893311050037