Radiosynthesis and biodistribution of 99mTc-tricarbonyl complex of temafloxacin dithiocarbamate: a potential Streptococci pneumoniae infection radiotracer
In the current investigation the complexation of derivatized temafloxacin with 99m Tc using [ 99m Tc(CO) 3 (H 2 O) 3 ] + precursor was assessed. The tricarbonyl complex of the temafloxacin dithiocarbamate ( 99m Tc(CO) 3 -TAND) was characterized in terms of radiochemical purity (RCP) yield in saline,...
Saved in:
Published in: | Journal of radioanalytical and nuclear chemistry 2011-05, Vol.288 (2), p.411-416 |
---|---|
Main Authors: | , |
Format: | Article |
Language: | eng |
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | In the current investigation the complexation of derivatized temafloxacin with
99m
Tc using [
99m
Tc(CO)
3
(H
2
O)
3
]
+
precursor was assessed. The tricarbonyl complex of the temafloxacin dithiocarbamate (
99m
Tc(CO)
3
-TAND) was characterized in terms of radiochemical purity (RCP) yield in saline, in vitro radiochemical stability in serum, in vitro binding with
Streptococci pneumoniae
and biodistribution in male Wister rats (MWR) artificially infected with living and heat killed
Streptococci pneumoniae.
The
99m
Tc(CO)
3
-TAND complex showed 98.10 ± 15% RCP value at 30 min of the reconstitution and remained more than 90% stable up to 120 min in normal saline at room temperature. In serum a stable behavior with the appearance of 15.30% unwanted side product up to 16 h of incubation was observed. A saturated in vitro binding with
Streptococci pneumoniae
was observed. The complex showed almost six times higher uptake in the infected muscle as compared to the inflamed and normal muscles of the MWR infected with living
Streptococci pneumoniae.
Insignificant difference in the uptake of the tracer in the infected, inflamed and normal muscles of the MWR infected with heat killed
Streptococci pneumoniae
was noted. Based on the elevated RCP in saline, in vitro stability in serum at 37 °C, saturated in vitro binding with pathogens and better biodistribution behavior with higher accumulation of the tracer in target organs confirmed the suitability of the
99m
Tc(CO)
3
-TAND complex as promising infection radiotracer. |
---|---|
ISSN: | 0236-5731 1588-2780 |