Radiolabeling of gemifloxacin with technetium-99m and biological evaluation in artificially Streptococcus pneumoniae infected rats
In the current investigation complexation of the gemifloxacin (GIN) with technetium-99 m ( 99m Tc) and its biological evaluation in artificially Streptococcus pneumoniae (S. pneumoniae) infected rats was assessed as potential S. pneumoniae infection radiotracer. Radiochemically the 99m Tc-GIN comple...
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Published in: | Journal of radioanalytical and nuclear chemistry 2011-04, Vol.288 (1), p.307-312 |
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Main Authors: | , |
Format: | Article |
Language: | eng |
Subjects: | |
Online Access: | Get full text |
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Summary: | In the current investigation complexation of the gemifloxacin (GIN) with technetium-99 m (
99m
Tc) and its biological evaluation in artificially
Streptococcus pneumoniae (S. pneumoniae)
infected rats was assessed as potential
S. pneumoniae
infection radiotracer. Radiochemically the
99m
Tc-GIN complex was further analyzed in terms of stability in saline, in vitro stability in serum at 37 °C, in vitro binding with
S. pneumoniae
and biodistribution in artificially
S. pneumoniae
(living and heat killed) infected rats. The complex was found 97.25 ± 0.25% radiochemically stable in saline at 30 min after reconstitution. The stability of the
99m
Tc-GIN complex was decreased to 90.50 ± 0.20% within 240 min after reconstitution. In serum the
99m
Tc-GIN complex showed stable profile with the appearance of 18.85% free tracer within 16 h of incubation. The
99m
Tc-GIN complex showed saturated in vitro binding with
S. pneumoniae
after different intervals. Almost five fold uptake was observed in living
S. pneumoniae
infected muscle of the rats as compared to the inflamed and normal muscle. No significant difference in the uptake of heat killed
S. pneumoniae
infected, inflamed and normal muscles of the rats. The high RCP yield in saline, in vitro permanence in serum, in vitro binding with living
S. pneumoniae
and biodistribution in artificially
S. pneumoniae
infected rats we recommend the
99m
Tc-GIN as potential
S. pneumoniae
infection radiotracer. |
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ISSN: | 0236-5731 1588-2780 |