Expression of a neurotoxin gene improves the insecticidal activity of Spodoptera litura nucleopolyhedrovirus (SpltNPV)

SpltNPV-C3 is a novel nucleopolyhedrovirus (NPV) screened from 189 wild Spodoptera litura nucleopolyhedrovirus (SpltNPV) clones collected throughout Japan and has high potential insecticidal activity against S. litura. In this study, we constructed two recombinant SpltNPVs, SpltNPV-Δ egt and SpltNPV...

Full description

Saved in:

Bibliographic Details
Published in:	Virus research 2011-07, Vol.159 (1), p.51-56
Main Authors:	Tang, Xin-Xin, Sun, Xing-Lu, Pu, Guan-Qin, Wang, Wen-Bing, Zhang, Chuan-Xi, Zhu, Jiang
Format:	Article
Language:	eng
Subjects:	Animals Baculovirus Bioassay bioassays BmK ITa1 Buthus martensii clones egt genes Genetic Engineering insecticidal properties instars Japan Larva - virology larvae lethal concentration 50 loci neurotoxins Neurotoxins - biosynthesis Neurotoxins - genetics Nuclear polyhedrosis virus Nucleopolyhedrovirus - pathogenicity Pest Control, Biological - methods population Recombination, Genetic SpltNPV Spodoptera - virology Spodoptera litura Spodoptera litura nucleopolyhedrovirus Survival Analysis Virulence viruses
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	SpltNPV-C3 is a novel nucleopolyhedrovirus (NPV) screened from 189 wild Spodoptera litura nucleopolyhedrovirus (SpltNPV) clones collected throughout Japan and has high potential insecticidal activity against S. litura. In this study, we constructed two recombinant SpltNPVs, SpltNPV-Δ egt and SpltNPV-Δ egt-BmK, which were characterized by the disruption of the UDP-glucosyltransferase gene ( egt) only and by replacing the egt locus with the Buthus martensi insect-specific toxin gene ( BmK ITa1), respectively. The insecticidal activity of these two recombinant viruses was evaluated by 50% lethal time (LT 50) and 50% lethal concentration (LC 50) using third instar S. litura larvae. Bioassays showed that the LT 50 of SpltNPV-Δ egt was reduced by 25% at a dose of 10 9 Occlusion bodies (OBs)/ml compared to the wild-type SpltNPV. However, the LC 50 values did not change. The SpltNPV-Δ egt-BmK reduced LT 50 by 28% at the highest dose of OBs (10 9 OBs/ml) compared to the wild-type SpltNPV, and the LC 50 was nearly an order of magnitude lower than those of the wild-type SpltNPV and SpltNPV-Δ egt. However, there was no discernable difference in the LT 50 between SpltNPV-Δ egt-BmK and SpltNPV-Δ egt when the 3rd instars of S. litura were fed a dose of 10 8 or 10 9 OBs/ml. The results suggest that these two recombinant forms of SpltNPV provide further opportunities to develop these viruses into commercially viable products to control S. litura populations.
ISSN:	0168-1702 1872-7492