Similarity of rpoB gene sequences of sucrose-fermenting and non-fermenting Corynebacterium diphtheriae strains

During the last decades, the majority of Brazilian Corynebacterium diphtheriae isolates were shown to be capable to metabolize sucrose, sometimes leading to erroneous identification as a non-diphtheric Corynebacterium species. The sequencing of the polymorphic region of the RNA polymerase beta subun...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2011-03, Vol.99 (3), p.733-737
Main Authors: Hirata, R. Jr, Pacheco, L. G, Soares, S. C, Santos, L. S, Moreira, L. O, Sabbadini, P. S, Santos, C. S, Miyoshi, A, Azevedo, V. A, Mattos-Guaraldi, A. L
Format: Article
Language:English
Subjects:
Bacteria
Bacterial diseases
Bacterial Proteins - classification
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Biomedical and Life Sciences
Biotypes
Contaminants
Corynebacterium
Corynebacterium diphtheriae
Corynebacterium diphtheriae - genetics
Corynebacterium diphtheriae - metabolism
Data processing
Diphtheria
DNA-directed RNA polymerase
Ent and stomatologic bacterial diseases
Fermentation
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Infectious diseases
Life Sciences
Medical Microbiology
Medical sciences
Microbiology
Miscellaneous
Phylogeny
Plant Sciences
RNA polymerase
rpoB gene
RpoB protein
Short Communication
Soil Science & Conservation
Sucrose
Sucrose - metabolism
Sucrose-fermenting biotype
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Summary:During the last decades, the majority of Brazilian Corynebacterium diphtheriae isolates were shown to be capable to metabolize sucrose, sometimes leading to erroneous identification as a non-diphtheric Corynebacterium species. The sequencing of the polymorphic region of the RNA polymerase beta subunit-encoding gene (rpoB) is an important taxonomic tool for identification of corynebacteria. The present study aimed to investigate the rpoB gene polymorphic features of sucrose-fermenting and non sucrose-fermenting strains. The results showed that sucrose-fermenting strains presented rpoB gene polymorphic regions with more than 98% similarity with the sequences deposited in the gene bank corresponding to non sucrose-fermenting strains. Data indicate that sucrose-fermenting isolates may act as a variant of C. diphtheriae biotype mitis. In addition we alert that sucrose-fermenting strains should not be discarded as contaminants mainly in countries where the possibility of isolation of this variant is higher.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-010-9519-0