Effect of Lactobacillus plantarum LP-Onlly on gut flora and colitis in interleukin-10 knockout mice

Background and Aim:  Probiotics are used in the therapy of inflammatory bowel disease. This study aimed to determine the effects of probiotic Lactobacillus plantarum LP‐Onlly (LP) on gut flora and colitis in interleukin‐10 knockout (IL‐10−/−) mice, a model of spontaneous colitis. Methods:  IL‐10−/−...

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Published in:Journal of gastroenterology and hepatology 2011-02, Vol.26 (2), p.405-411
Main Authors: Xia, Yang, Chen, Hong-Qi, Zhang, Min, Jiang, Yan-Qun, Hang, Xiao-Min, Qin, Huan-Long
Format: Article
Language:English
Subjects:
Adjuvants
Administration, Oral
Age
Animal models
Animals
Bifidobacterium - growth & development
Biological and medical sciences
Clostridium perfringens
Clostridium perfringens - growth & development
Coliforms
Colitis
Colitis - genetics
Colitis - immunology
Colitis - microbiology
Colitis - pathology
Colitis - prevention & control
Colon
Colon - immunology
Colon - microbiology
Colon - ultrastructure
Digestive tract
Disease Models, Animal
Diseases of the digestive system
Enterococcus - growth & development
Feces
Feces - microbiology
Female
Gastroenterology. Liver. Pancreas. Abdomen
gut flora
inflammatory bowel disease
Inflammatory bowel diseases
Injuries
Interleukin 10
Interleukin-10 - deficiency
Interleukin-10 - genetics
Intestine
Lactobacillus
Lactobacillus plantarum
Lactobacillus plantarum - growth & development
Medical sciences
Mice
Mice, 129 Strain
Mice, Knockout
Microflora
Oral administration
Other diseases. Semiology
probiotics
Probiotics - administration & dosage
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Severity of Illness Index
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Time Factors
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Summary:Background and Aim:  Probiotics are used in the therapy of inflammatory bowel disease. This study aimed to determine the effects of probiotic Lactobacillus plantarum LP‐Onlly (LP) on gut flora and colitis in interleukin‐10 knockout (IL‐10−/−) mice, a model of spontaneous colitis. Methods:  IL‐10−/− and wild‐type mice were used at 8 weeks of age and LP by gavage was administered at a dose of 109 cells/day per mice for 4 weeks. Mice were maintained for another one week without LP treatment. The colonic tissues were collected for histological and ultrastructural analysis at death after 4 weeks treatment of LP, and the feces were collected at 1‐week intervals throughout the experiment for the analysis of gut flora and LP using selective culture‐based techniques. Results:  Compared with control mice, IL‐10−/− mice developed a severe intestinal inflammation and tissue damage, and had an abnormal composition of gut microflora. LP administration attenuated colitis with the decreased inflammatory scoring and histological injury in the colon of IL‐10−/− mice. In addition, LP administration increased the numbers of beneficial total bifidobacteria and lactobacilli, and decreased the numbers of potential pathogenic enterococci and Clostridium perfringens, although the decrease of coliforms was not significant after LP treatment in IL‐10−/− mice. Conclusions:  Oral administration of LP was effective in the treatment of colitis, with the direct modification of gut microflora in IL‐10−/− mice. This probiotic strain could be used as a potential adjuvant in the therapy of inflammatory bowel disease, although further studies are required in human.
ISSN:0815-9319
1440-1746
DOI:10.1111/j.1440-1746.2010.06498.x