ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China

Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae has spread rapidly in China. In this study, we aimed to investigate the molecular epidemiology of KPC-producing K. pneumoniae isolates in China. Ninety-five carbapenem-resistant K. pneumoniae isolates from 13 hospitals in nine...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2011-02, Vol.66 (2), p.307-312
Main Authors: Qi, Yan, Wei, Zeqing, Ji, Shujuan, Du, Xiaoxing, Shen, Ping, Yu, Yunsong
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacterial Typing Techniques
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Biological and medical sciences
Blotting, Southern
China - epidemiology
Drug resistance
Drug Resistance, Multiple, Bacterial - genetics
Epidemiology
Genes
Gram-negative bacteria
Hospitals
Humans
Klebsiella Infections - drug therapy
Klebsiella Infections - epidemiology
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Medical sciences
Microbial Sensitivity Tests
Molecular Epidemiology
Multilocus Sequence Typing
Pharmacology. Drug treatments
Plasmids
Plasmids - drug effects
Plasmids - genetics
Polymerase Chain Reaction
Restriction Mapping
Studies
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Summary:Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae has spread rapidly in China. In this study, we aimed to investigate the molecular epidemiology of KPC-producing K. pneumoniae isolates in China. Ninety-five carbapenem-resistant K. pneumoniae isolates from 13 hospitals in nine cities covering five provinces in China were analysed. Antibiotic susceptibility was determined by the Etest. Multilocus sequence typing (MLST) and PFGE were used for epidemiological analysis. The genetic structure around bla(KPC) and the encoding genes of extended-spectrum β-lactamases and plasmid-mediated AmpC enzymes were detected by PCR and sequencing. Plasmids were analysed by transformation, restriction and Southern blot. All isolates harboured the bla(KPC-2) gene. Seven sequence types (STs) were obtained. The dominant clone was ST11 (61/95), which was identified in isolates from Zhejiang province (four hospitals in Hangzhou and one hospital in Ningbo), Jiangsu province (one hospital in Nanjing) and Anhui province (one hospital in Hefei). Isolates with ST11 showed >80% similarity in PFGE patterns. Plasmids from 14 selected transformants, their original isolates representing different STs and/or regions, had a diversity of HindIII restriction maps. The dominant clone of KPC-producing K. pneumoniae in China is ST11, which is closely related to ST258, which has been reported worldwide.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dkq431