Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

Zeocin is a member of bleomycin/phleomycin family of antibiotics isolated from Streptomyces verticullus. This unique radiomimetic antibiotic is known to bind to DNA and induce oxidative stress in different organisms producing predominantly single- and double- strand breaks, as well as a DNA base los...

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Bibliographic Details
Published in:Neoplasma 2012, Vol.59 (1), p.62-69
Main Authors: Hudecova, A, Hasplova, K, Kellovska, L, Ikreniova, M, Miadokova, E, Galova, E, Horvathova, E, Vaculcikova, D, Gregan, F, Dusinska, M
Format: Article
Language:English
Subjects:
Adaptation, Biological - drug effects
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - antagonists & inhibitors
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - toxicity
Armoracia - chemistry
Bleomycin - administration & dosage
Bleomycin - antagonists & inhibitors
Bleomycin - pharmacology
Bleomycin - toxicity
Comet Assay
DNA Breaks, Double-Stranded - drug effects
DNA Breaks, Single-Stranded - drug effects
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Drug Interactions
Electrophoresis, Agar Gel
Gene Expression Regulation - drug effects
Gentiana - chemistry
Humans
Lymphocytes - cytology
Lymphocytes - drug effects
Methanol
Mutagenicity Tests
Oxidative Stress - drug effects
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plant Roots - chemistry
Solvents
Water
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Summary:Zeocin is a member of bleomycin/phleomycin family of antibiotics isolated from Streptomyces verticullus. This unique radiomimetic antibiotic is known to bind to DNA and induce oxidative stress in different organisms producing predominantly single- and double- strand breaks, as well as a DNA base loss resulting in apurinic/apyrimidinic (AP) sites. The aim of this study was to induce an adaptive response (AR) by zeocin in freshly isolated human lymphocytes from blood and to observe whether plant extracts could modulate this response. The AR was evaluated by the comet assay. The optimal conditions for the AR induction and modulation were determined as: 2 h-intertreatment time (in PBS, at 4°C) given after a priming dose (50 µg/ml) of zeocin treatment. Genotoxic impact of zeocin to lymphocytes was modulated by plant extracts isolated from Gentiana asclepiadea (methanolic and aqueous haulm extracts, 0.25 mg/ml) and Armoracia rusticana (methanolic root extract, 0.025 mg/ml). These extracts enhanced the AR and also decreased DNA damage caused by zeocin (after 0, 1 and 4 h-recovery time after the test dose of zeocin application) to more than 50%. These results support important position of plants containing many biologically active compounds in the field of pharmacology and medicine.
ISSN:0028-2685
DOI:10.4149/neo_2012_01_008