Neurobiological activity of Parawixin 10, a novel anticonvulsant compound isolated from Parawixia bistriata spider venom (Araneidae: Araneae)

Abstract The neurobiological activity of Parawixin 10, isolated from Parawixia bistriata spider venom, was investigated. Cannulas were implanted in the lateral ventricles of Wistar rats (200–250 g, n = 6–8 per group) to perform anticonvulsant and behavioral assays, and synaptosomes from cerebral cor...

Full description

Saved in:
Bibliographic Details
Published in:Epilepsy & behavior 2011-10, Vol.22 (2), p.158-164
Main Authors: Fachim, Helene Aparecida, Cunha, Alexandra Olimpio Siqueira, Pereira, Adriana Colsera, Beleboni, René Oliveira, Gobbo-Neto, Leonardo, Lopes, Norberto Peporine, Coutinho-Netto, Joaquim, dos Santos, Wagner Ferreira
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Anticonvulsant
Anticonvulsants - chemistry
Anticonvulsants - therapeutic use
Araneae
Araneidae
Ataxia - drug therapy
Ataxia - etiology
Cerebral Cortex - ultrastructure
Chromatography, High Pressure Liquid
Disease Models, Animal
Dose-Response Relationship, Drug
Excitatory Amino Acid Agonists - toxicity
gamma-Aminobutyric Acid - metabolism
Glutamate
Glutamic Acid - metabolism
Glycine
Glycine - drug effects
Kainic Acid - toxicity
Male
Motor Activity - drug effects
N-Methylaspartate - toxicity
Neurology
Parawixia bistriata
Parawixin 10
Rats
Rats, Wistar
Reaction Time - drug effects
Seizures - chemically induced
Seizures - complications
Seizures - drug therapy
Seizures - pathology
Spider venom
Spider Venoms - chemistry
Spider Venoms - therapeutic use
Synaptosomes - drug effects
Synaptosomes - metabolism
Tritium - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The neurobiological activity of Parawixin 10, isolated from Parawixia bistriata spider venom, was investigated. Cannulas were implanted in the lateral ventricles of Wistar rats (200–250 g, n = 6–8 per group) to perform anticonvulsant and behavioral assays, and synaptosomes from cerebral cortices of male Wistar rats were used for neurochemical studies. The results indicate that pretreatment with Parawixin 10 prevents the onset of seizures induced with kainic acid, N -methyl-D-aspartate, and pentylenetetrazole in a dose–response manner. Lower doses of Parawixin 10 significantly increased the latency to onset of kainic acid-, pentylenetetrazole-, and N -methyl-D-aspartate-induced seizures. There were maximum increases of 79% in L-[3 H]glutamine uptake and 40% in [3 H]glycine uptake; [3 H]GABA uptake did not change. The findings demonstrate that this novel compound from P. bistriata venom exerts a pharmacological effect on the glutamatergic and glycinergic systems.
ISSN:1525-5050
1525-5069
DOI:10.1016/j.yebeh.2011.05.008