No mutation but high mRNA expression of Coxsackie-Adenovirus Receptor was observed in both dilated and ischemic cardiomyopathy

Abstract The most common causes of acute myocarditis and the possible consequence of dilated cardiomyopathy are virus infections. The receptor of the two most common viruses connected to these myocardial diseases was identified as Coxsackie-Adenovirus Receptor. The purpose of this study was to asses...

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Bibliographic Details
Published in:Forensic science international 2011-10, Vol.212 (1), p.47-50
Main Authors: Tatrai, Eniko, Bedi, Katalin, Kovalszky, Ilona, Hartyanszky, Istvan, Laszik, Andras, Acsady, Gyorgy, Sotonyi, Peter, Hubay, Marta
Format: Article
Language:English
Subjects:
Adenoviruses
Adolescent
Adult
Biological and medical sciences
Cardiomyopathy
Cardiomyopathy, Dilated - pathology
Cardiomyopathy, Dilated - virology
Cardiovascular disease
Case-Control Studies
Coxsackie and Adenovirus Receptor-Like Membrane Protein
Coxsackie-Adenovirus Receptor
Coxsackievirus Infections - diagnosis
Coxsackievirus Infections - pathology
Deoxyribonucleic acid
DNA
DNA, Viral - analysis
Female
Forensic medicine
Forensic Medicine - methods
Forensic sciences
Gene Expression Regulation, Viral
General aspects
Genes
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Middle Aged
Mutation
Myocardial Ischemia - pathology
Myocardial Ischemia - virology
Myocarditis
Myocarditis - pathology
Myocarditis - virology
Myocardium - pathology
Pathology
Proteins
Public health. Hygiene
Public health. Hygiene-occupational medicine
Real-Time Polymerase Chain Reaction
Receptors, Virus - genetics
Receptors, Virus - metabolism
Reference Values
Ribonucleic acid
RNA
RNA, Messenger - analysis
Rodents
Up-Regulation
Virus
Young Adult
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Summary:Abstract The most common causes of acute myocarditis and the possible consequence of dilated cardiomyopathy are virus infections. The receptor of the two most common viruses connected to these myocardial diseases was identified as Coxsackie-Adenovirus Receptor. The purpose of this study was to assess Coxsackie-Adenovirus Receptor mRNA expression in the myocardium and search for mutations in the Coxsackie-Adenovirus Receptor gene to compare dilated, inflammatory and ischemic cardiomyopathy with control group. All the myocardial samples were obtained from 35 explanted hearts during heart transplantation, than DNA and RNA were isolated from the muscle samples. cDNA was generated from RNA using reverse transcription, and real-time PCR was performed with relative quantification by β-actin gene as endogenous control. Using DNA extracted from the myocardial samples, we sequenced all the seven exons of the Coxsackie-Adenovirus Receptor gene. Coxsackie-Adenovirus Receptor mRNA expression was higher in both ischemic and dilated cardiomyopathy groups than in inflammatory cardiomyopathy and healthy control groups. Sequencing of CAR gene showed no sign of mutation. Therefore, the sequences result of CAR exons did not show any mutation or polymorphism, that explains a determinant role of CAR in dilated or ischemic CM. Our results suggest that high mRNA expression of Coxsackie-Adenovirus Receptor may support its role in regeneration of the damaged myocardium rather than having any role in viral mediated heart disease.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2011.05.010