In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea

Abstract The purpose of this study was to quantify the biofilm formations of various methicillin-resistant Staphylococcus aureus (MRSA) clones (ST1, ST5, ST72, ST89, ST239, and ST254) and to evaluate the in vitro activities of antibiotics. Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biof...

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Published in:Diagnostic microbiology and infectious disease 2011-05, Vol.70 (1), p.112-118
Main Authors: Cha, Jeong-Ok, Park, Yong-Keun, Lee, Yeong Seon, Chung, Gyung Tae
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bactericidal activity
Bacteriology
Biofilms - growth & development
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Humans
Infectious Disease
Infectious diseases
Internal Medicine
MBC
Medical sciences
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - growth & development
Methicillin-Resistant Staphylococcus aureus - isolation & purification
MIC
Microbial Sensitivity Tests
Microbial Viability - drug effects
Microbiology
Miscellaneous
MRSA
Republic of Korea
Staphylococcal Infections - microbiology
Staphylococcus aureus
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Summary:Abstract The purpose of this study was to quantify the biofilm formations of various methicillin-resistant Staphylococcus aureus (MRSA) clones (ST1, ST5, ST72, ST89, ST239, and ST254) and to evaluate the in vitro activities of antibiotics. Of 86 MRSA isolates tested, 44 isolates (51.2 %) formed biofilms, including 8 strong biofilm producers (7 isolates of ST72 and 1 isolate of ST89). The planktonic MICs, minimal bactericidal concentrations, and minimum biofilm eradication concentrations (MBECs) of 6 antibiotics (clindamycin, gentamicin, linezolid, vancomycin, daptomycin, and tigecycline) were measured for 8 MRSA isolates. The planktonic isolates were susceptible to all agents, with daptomycin being the most effective, followed by gentamicin and vancomycin. However, clindamycin, linezolid, and tigecycline showed bacteriostatic activity against most of the isolates. The biofilms were resistant to high concentrations of most drugs. However, the MBEC values of daptomycin and tigecycline were lower overall than that of other antibiotics. Microscopy studies verified that daptomycin was the most effective drug used in adhesive biofilms, while gentamicin and tigecycline exhibited some bactericidal efficacy. Our results demonstrated that ST72 and ST89 form strong biofilms that may cause problems in hospital setting, and daptomycin, gentamicin, and tigecycline may be choice therapeutics against biofilm-mediated S. aureus infections.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2010.11.018