Analysis of Chromosome Translocations Involving MML by Hybridization with an Oligonucleotide Microarray

Identification of chromosome rearrangements is of importance for exact diagnosis, risk assessment, and therapy in blood malignancies. A new method was proposed for rapid and accurate identification of leukemia forms caused by chromosome rearrangements involving MLL (11q23). The method combines rever...

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Bibliographic Details
Published in:Molecular biology (New York) 2004-05, Vol.38 (3), p.376-382
Main Authors: Mityaeva, O. N., Nasedkina, T. V., Zharinov, V. S., Isaeva, E. A., Turigin, A. Yu, Chupeeva, V. V., Kreindlin, E. Ya, Mirzabekov, A. D.
Format: Article
Language:English
Subjects:
Chromosomes
Risk assessment
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Summary:Identification of chromosome rearrangements is of importance for exact diagnosis, risk assessment, and therapy in blood malignancies. A new method was proposed for rapid and accurate identification of leukemia forms caused by chromosome rearrangements involving MLL (11q23). The method combines reverse transcription-multiplex PCR and hybridization with an oligonucleotide microarray. The microarray was designed to detect the five most common MLL rearrangements: t(4; 11) MLL/AF4, t(9; 11) MLL/AF9, t(11; 19) MLL/ELL, t(11; 19) MLL/ENL, and dup11 MLL/MLL. With clinical specimens, the method was shown to efficiently identify the chromosome translocations in leukemia patients.
ISSN:0026-8933
1608-3245
DOI:10.1023/B:MBIL.0000032208.13834.8b