Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds

For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as template...

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Bibliographic Details
Published in:Acta biomaterialia 2009-07, Vol.5 (6), p.2063-2071
Main Authors: Kanczler, Janos M., Mirmalek-Sani, Sayed-Hadi, Hanley, Neil A., Ivanov, Alexander L., Barry, John J.A., Upton, Clare, Shakesheff, Kevin M., Howdle, Steven M., Antonov, Eugeuni N., Bagratashvili, Victor N., Popov, Vladimir K., Oreffo, Richard O.C.
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Cell Culture Techniques - methods
Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Feasibility Studies
Femur - cytology
Femur - embryology
Hot Temperature
Human fetal femur-derived cells
Humans
Lactic Acid - chemistry
Lasers
Materials Testing
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis
Osteogenesis - physiology
Polyesters
Polymers - chemistry
Surface Properties
Surface selective laser sintering
Tissue engineering
Tissue Engineering - methods
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Summary:For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly( d, l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2009.03.010