Supranutritional vitamin E supplementation in pigs: Influence on subcellular deposition of α-tocopherol and on oxidative stability by conventional and derivative spectrophotometry

The influence of three levels of vitamin E (30, 200 and 1000mg kg −1) in the diet of pigs on the subcellular deposition of α-tocopherol in muscle and on the oxidative stability determined by conventional and first derivative Spectrophotometry was studied. The content of α-tocopherol in m. gluteo bic...

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Bibliographic Details
Published in:Meat science 1997-11, Vol.47 (3), p.301-310
Main Authors: Wen, J., Morrissey, P.A., Buckley, D.J., Sheehy, P.J.A.
Format: Article
Language:English
Subjects:
Animal productions
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Terrestrial animal productions
Vertebrates
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Summary:The influence of three levels of vitamin E (30, 200 and 1000mg kg −1) in the diet of pigs on the subcellular deposition of α-tocopherol in muscle and on the oxidative stability determined by conventional and first derivative Spectrophotometry was studied. The content of α-tocopherol in m. gluteo biceps and in mitochondrial and microsomal fractions of the muscle significantly increased ( p < 0.01) with increasing levels of dietary vitamin E. Concentrations of α-tocopherol in muscle, mitochondria and microsomes of pigs fed diet supplemented with 1000 mg kg −1 α-tocopheryl acetate were 3.2-, 6.1- and 5.6-fold greater, respectively, than those in their counterparts from the control animals. These differences in α-tocopherol concentration in the subcellular fractions and intact muscle resulted in enhanced stability of the membranes and the tissue when exposed to iron-ascorbate induced peroxidation. When lipid oxidation in the same samples was further measured by the first derivative method, the resultant MDA-TBA values were 59–69% lower in tissue samples, 16–19% lower in mitochondria and 6–9% lower in microsomes than the conventional TBARS values.
ISSN:0309-1740
1873-4138
DOI:10.1016/S0309-1740(97)00062-4