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Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma
The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little i...
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| Published in: | Gene 2011-07, Vol.481 (1), p.41-47 |
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| container_title | Gene |
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| creator | Ng, Chor-Fung Zhou, Wayne Jun-Wei Ng, Patrick Kwok-Shing Li, Man-Shan Ng, Yuen-Keng Lai, Paul Bo-San Tsui, Stephen Kwok-Wing |
| description | The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human
FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little is known about the functional role of the different variants and the mechanism of gene regulation. In the present study, we characterized the different alternative spliced transcripts of
FHL2 by
in silico analysis and RT-PCR analysis. A novel transcript variant was identified. The
FHL2 gene produces transcripts by different 5′ exons, which may be responsible for tissue-specific regulation. To study the mechanism of
FHL2 gene regulation, the potential promoter region was investigated. We have identified a functional promoter region upstream of the transcription start site. Deletion mutation analysis of 5′ flanking region showed that the fragment from −
138 to +
292
bp have positive regulatory effect. We identified the binding sites of Pax-5/ZF5 in this region and found that Pax-5 and ZF5 expression in HCC samples had a significant positive correlation with FHL2 expression, suggesting a possible role for these transcription factors in the regulation of
FHL2 expression. |
| doi_str_mv | 10.1016/j.gene.2011.04.005 |
| format | article |
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FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little is known about the functional role of the different variants and the mechanism of gene regulation. In the present study, we characterized the different alternative spliced transcripts of
FHL2 by
in silico analysis and RT-PCR analysis. A novel transcript variant was identified. The
FHL2 gene produces transcripts by different 5′ exons, which may be responsible for tissue-specific regulation. To study the mechanism of
FHL2 gene regulation, the potential promoter region was investigated. We have identified a functional promoter region upstream of the transcription start site. Deletion mutation analysis of 5′ flanking region showed that the fragment from −
138 to +
292
bp have positive regulatory effect. We identified the binding sites of Pax-5/ZF5 in this region and found that Pax-5 and ZF5 expression in HCC samples had a significant positive correlation with FHL2 expression, suggesting a possible role for these transcription factors in the regulation of
FHL2 expression.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2011.04.005</identifier><identifier>PMID: 21540083</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>5' Flanking Region ; Binding Sites ; Carcinoma, Hepatocellular - genetics ; Cell Line, Tumor ; exons ; FHL2 ; Gene Expression Regulation ; genes ; heart ; hepatoma ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; LIM-Homeodomain Proteins ; Liver cancer ; Liver Neoplasms - genetics ; Muscle Proteins - genetics ; Muscle Proteins - metabolism ; mutation ; Promoter ; promoter regions ; Promoter Regions, Genetic ; Protein Isoforms ; Reverse Transcriptase Polymerase Chain Reaction ; Splice variants ; Transcription factor binding sites ; transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Gene, 2011-07, Vol.481 (1), p.41-47</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-450fe59fe284c4fe31d54903e4851a60d2c5c1a11e7426f625f9724e55f7a05b3</citedby><cites>FETCH-LOGICAL-c411t-450fe59fe284c4fe31d54903e4851a60d2c5c1a11e7426f625f9724e55f7a05b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21540083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng, Chor-Fung</creatorcontrib><creatorcontrib>Zhou, Wayne Jun-Wei</creatorcontrib><creatorcontrib>Ng, Patrick Kwok-Shing</creatorcontrib><creatorcontrib>Li, Man-Shan</creatorcontrib><creatorcontrib>Ng, Yuen-Keng</creatorcontrib><creatorcontrib>Lai, Paul Bo-San</creatorcontrib><creatorcontrib>Tsui, Stephen Kwok-Wing</creatorcontrib><title>Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma</title><title>Gene</title><addtitle>Gene</addtitle><description>The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human
FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little is known about the functional role of the different variants and the mechanism of gene regulation. In the present study, we characterized the different alternative spliced transcripts of
FHL2 by
in silico analysis and RT-PCR analysis. A novel transcript variant was identified. The
FHL2 gene produces transcripts by different 5′ exons, which may be responsible for tissue-specific regulation. To study the mechanism of
FHL2 gene regulation, the potential promoter region was investigated. We have identified a functional promoter region upstream of the transcription start site. Deletion mutation analysis of 5′ flanking region showed that the fragment from −
138 to +
292
bp have positive regulatory effect. We identified the binding sites of Pax-5/ZF5 in this region and found that Pax-5 and ZF5 expression in HCC samples had a significant positive correlation with FHL2 expression, suggesting a possible role for these transcription factors in the regulation of
FHL2 expression.</description><subject>5' Flanking Region</subject><subject>Binding Sites</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Cell Line, Tumor</subject><subject>exons</subject><subject>FHL2</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>heart</subject><subject>hepatoma</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>LIM-Homeodomain Proteins</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Muscle Proteins - genetics</subject><subject>Muscle Proteins - metabolism</subject><subject>mutation</subject><subject>Promoter</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Isoforms</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Splice variants</subject><subject>Transcription factor binding sites</subject><subject>transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS0EotvCH-AAvnFK8Dh24khc0IpSpJV6aHu2XGe869XGCXZSFX49jtJypL6MZH3vzeg9Qj4AK4FB_eVY7jFgyRlAyUTJmHxFNqCatmCsUq_JhlWNKgCgPSPnKR1ZflLyt-SMgxSMqWpD-u3BRGMnjP6PmfwQ6ODoYe5NoJdXO06naEKy0Y8TfTDRmzAlakJHl80UH8eIKS2qiPv5tBr4QA84mmmweDrlz0itidaHoTfvyBtnTgnfP80Lcnf5_XZ7Veyuf_zcftsVVgBMhZDMoWwdciWscFhBJ0XLKhRKgqlZx620YACwEbx2NZeubbhAKV1jmLyvLsjn1XeMw68Z06R7n5ZzTMBhTrplvFK8BvkiqepWtrzhTSb5Sto4pBTR6TH63sTfGphe-tBHvaSilz40EzqHnUUfn-zn-x67f5LnAjLwaQWcGbTZR5_03U12kLmrqqmUysTXlcAc2IPHqJP1GCx2PqKddDf4_13wF1F0pcc</recordid><startdate>20110715</startdate><enddate>20110715</enddate><creator>Ng, Chor-Fung</creator><creator>Zhou, Wayne Jun-Wei</creator><creator>Ng, Patrick Kwok-Shing</creator><creator>Li, Man-Shan</creator><creator>Ng, Yuen-Keng</creator><creator>Lai, Paul Bo-San</creator><creator>Tsui, Stephen Kwok-Wing</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110715</creationdate><title>Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma</title><author>Ng, Chor-Fung ; Zhou, Wayne Jun-Wei ; Ng, Patrick Kwok-Shing ; Li, Man-Shan ; Ng, Yuen-Keng ; Lai, Paul Bo-San ; Tsui, Stephen Kwok-Wing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-450fe59fe284c4fe31d54903e4851a60d2c5c1a11e7426f625f9724e55f7a05b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>5' Flanking Region</topic><topic>Binding Sites</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Cell Line, Tumor</topic><topic>exons</topic><topic>FHL2</topic><topic>Gene Expression Regulation</topic><topic>genes</topic><topic>heart</topic><topic>hepatoma</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>LIM-Homeodomain Proteins</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Muscle Proteins - genetics</topic><topic>Muscle Proteins - metabolism</topic><topic>mutation</topic><topic>Promoter</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Isoforms</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Splice variants</topic><topic>Transcription factor binding sites</topic><topic>transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, Chor-Fung</creatorcontrib><creatorcontrib>Zhou, Wayne Jun-Wei</creatorcontrib><creatorcontrib>Ng, Patrick Kwok-Shing</creatorcontrib><creatorcontrib>Li, Man-Shan</creatorcontrib><creatorcontrib>Ng, Yuen-Keng</creatorcontrib><creatorcontrib>Lai, Paul Bo-San</creatorcontrib><creatorcontrib>Tsui, Stephen Kwok-Wing</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, Chor-Fung</au><au>Zhou, Wayne Jun-Wei</au><au>Ng, Patrick Kwok-Shing</au><au>Li, Man-Shan</au><au>Ng, Yuen-Keng</au><au>Lai, Paul Bo-San</au><au>Tsui, Stephen Kwok-Wing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2011-07-15</date><risdate>2011</risdate><volume>481</volume><issue>1</issue><spage>41</spage><epage>47</epage><pages>41-47</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><notes>http://dx.doi.org/10.1016/j.gene.2011.04.005</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Article-2</notes><notes>ObjectType-Feature-1</notes><abstract>The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human
FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little is known about the functional role of the different variants and the mechanism of gene regulation. In the present study, we characterized the different alternative spliced transcripts of
FHL2 by
in silico analysis and RT-PCR analysis. A novel transcript variant was identified. The
FHL2 gene produces transcripts by different 5′ exons, which may be responsible for tissue-specific regulation. To study the mechanism of
FHL2 gene regulation, the potential promoter region was investigated. We have identified a functional promoter region upstream of the transcription start site. Deletion mutation analysis of 5′ flanking region showed that the fragment from −
138 to +
292
bp have positive regulatory effect. We identified the binding sites of Pax-5/ZF5 in this region and found that Pax-5 and ZF5 expression in HCC samples had a significant positive correlation with FHL2 expression, suggesting a possible role for these transcription factors in the regulation of
FHL2 expression.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21540083</pmid><doi>10.1016/j.gene.2011.04.005</doi><tpages>7</tpages></addata></record> |
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| ispartof | Gene, 2011-07, Vol.481 (1), p.41-47 |
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| language | eng |
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| subjects | 5' Flanking Region Binding Sites Carcinoma, Hepatocellular - genetics Cell Line, Tumor exons FHL2 Gene Expression Regulation genes heart hepatoma Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans LIM-Homeodomain Proteins Liver cancer Liver Neoplasms - genetics Muscle Proteins - genetics Muscle Proteins - metabolism mutation Promoter promoter regions Promoter Regions, Genetic Protein Isoforms Reverse Transcriptase Polymerase Chain Reaction Splice variants Transcription factor binding sites transcription factors Transcription Factors - genetics Transcription Factors - metabolism |
| title | Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma |
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