Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma

The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little i...

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Bibliographic Details
Published in:Gene 2011-07, Vol.481 (1), p.41-47
Main Authors: Ng, Chor-Fung, Zhou, Wayne Jun-Wei, Ng, Patrick Kwok-Shing, Li, Man-Shan, Ng, Yuen-Keng, Lai, Paul Bo-San, Tsui, Stephen Kwok-Wing
Format: Article
Language:English
Subjects:
5' Flanking Region
Binding Sites
Carcinoma, Hepatocellular - genetics
Cell Line, Tumor
exons
FHL2
Gene Expression Regulation
genes
heart
hepatoma
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Humans
LIM-Homeodomain Proteins
Liver cancer
Liver Neoplasms - genetics
Muscle Proteins - genetics
Muscle Proteins - metabolism
mutation
Promoter
promoter regions
Promoter Regions, Genetic
Protein Isoforms
Reverse Transcriptase Polymerase Chain Reaction
Splice variants
Transcription factor binding sites
transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human FHL2 gene expresses different transcripts which are known to differ only in the 5′UTR region. However, little is known about the functional role of the different variants and the mechanism of gene regulation. In the present study, we characterized the different alternative spliced transcripts of FHL2 by in silico analysis and RT-PCR analysis. A novel transcript variant was identified. The FHL2 gene produces transcripts by different 5′ exons, which may be responsible for tissue-specific regulation. To study the mechanism of FHL2 gene regulation, the potential promoter region was investigated. We have identified a functional promoter region upstream of the transcription start site. Deletion mutation analysis of 5′ flanking region showed that the fragment from − 138 to + 292 bp have positive regulatory effect. We identified the binding sites of Pax-5/ZF5 in this region and found that Pax-5 and ZF5 expression in HCC samples had a significant positive correlation with FHL2 expression, suggesting a possible role for these transcription factors in the regulation of FHL2 expression.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2011.04.005