Preferential elimination of chromosome 5R of rye in the progeny of 5R5D dimonosomics

Transmission of chromosome 5R of rye ( Secale cereale L.) and chromosome 5D of common wheat ( Triticum aestivum L.) through gametes of 5R5D dimonosomics (2 n = 42, 20W″ + 5R′ + 5D′) was studied. Chromosome 5R was found to have lower competitiveness as compared to 5D. Gametes with the rye chromosome...

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Published in:Russian journal of genetics 2011-08, Vol.47 (8), p.942-950
Main Authors: Silkova, O. G., Leonova, I. N., Krasilova, N. M., Dubovets, N. I.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedicine
Centromeres
Chromosome deletion
Chromosome translocations
Chromosomes
Competitiveness
Gametes
Human Genetics
Hybrids
Isochromosomes
Karyotypes
Microbial Genetics and Genomics
Plant Genetics
Polymerase chain reaction
Secale cereale
Triticum aestivum
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Summary:Transmission of chromosome 5R of rye ( Secale cereale L.) and chromosome 5D of common wheat ( Triticum aestivum L.) through gametes of 5R5D dimonosomics (2 n = 42, 20W″ + 5R′ + 5D′) was studied. Chromosome 5R was found to have lower competitiveness as compared to 5D. Gametes with the rye chromosome were two times less often involved in the formation of a progeny. The combined frequency of the karyotypes of wheat (5D5D) and wheat monosomics (5D) was 11.6-fold higher than the frequency of the karyotypes of substitution lines (5R5R) and monosomics for the rye chromosome (5R). The karyotypes of 10.38% of hybrid plants had aberrant 5R chromosomes with different translocations formed as a result of breakages in the centromere and in the proximal region of the long arm. Telocentrics for the short arm t5RS, i5RS isochromosomes, and chromosomes with a terminal deletion T5RS.5RL-del were identified. The absence of amplification of SSR markers mapped on 5RS and the detection of PCR products for a number of 5RL markers (including the genome-specific rye marker Xrms115) permitted nine plants carrying only the long arm of chromosome 5R to be revealed. Since t5RL telocentrics were not detected by the cytological analysis, the results obtained allow us to suggest the presence of small intercalary translocations of the long arm of chromosome 5R in chromosome 5D or in other wheat chromosomes.
ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795411080151