In vitro haploid and dihaploid production via unfertilized ovule culture

Haploids and doubled haploids are very important in plant breeding, enabling the time needed to produce homozygous lines to be shortened compared with conventional breeding. In the present review, emphasis is given to haploid induction through unfertilized ovule/ovary culture. Attention is given to...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2011-03, Vol.104 (3), p.311-319
Main Authors: Chen, Jin-Feng, Cui, Li, Malik, Ahmed Abbas, Mbira, Kere George
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Culture media
Developmental stages
Dihaploid
Genetics
Genotypes
Gynogenesis
Life Sciences
Plant breeding
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Pretreatment
Review
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Summary:Haploids and doubled haploids are very important in plant breeding, enabling the time needed to produce homozygous lines to be shortened compared with conventional breeding. In the present review, emphasis is given to haploid induction through unfertilized ovule/ovary culture. Attention is given to induction of haploid plants from female gametophyte culture through analysis of factors in the processes of gynogenesis, including genotype selection, stage of ovule development, pretreatment, and culture media containing nutritional components and phytohormones. The gynogenetic approach may be of great value in discovering novel genetic recombinations. Application of double haploids in genetics and plant breeding is also highlighted. This review also identifies some existing knowledge gaps where work may increase the efficiency of this process in different plant species.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-010-9874-6