Association mapping of late maturity α-amylase (LMA) activity in a collection of synthetic hexaploid wheat

Late maturity α-amylase (LMA) is a genetic defect of wheat which results in the production of α-amylase, shown as substandard falling numbers, in the absence of preharvest rain and under cool temperatures during ripening. The present study is an attempt to use a whole-genome scan with DArT markers t...

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Bibliographic Details
Published in:Molecular breeding 2010, Vol.26 (1), p.39-49
Main Authors: Emebiri, L. C, Oliver, J. R, Mrva, K, Mares, D
Format: Article
Language:English
Subjects:
absorbance
Agricultural production
alpha-amylase
Amylases
Association mapping
Biomedical and Life Sciences
Biotechnology
Bread
Chromosome 7
Chromosomes
Collection
DArT markers
Gene expression
Gene mapping
genes
genetic disorders
Heritability
hexaploidy
Isoenzymes
isozymes
Late maturity α-amylase
Life Sciences
Linkage disequilibrium
Mapping
Markers
Molecular biology
Optical density
phenotype
Phenotypes
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
rain
Ripening
Synthetic hexaploid wheat
temperature
Triticum aestivum
Wheat
α-Amylase
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Summary:Late maturity α-amylase (LMA) is a genetic defect of wheat which results in the production of α-amylase, shown as substandard falling numbers, in the absence of preharvest rain and under cool temperatures during ripening. The present study is an attempt to use a whole-genome scan with DArT markers to identify chromosomal regions influencing LMA in synthetic hexaploid wheat (SHW). A high heritability estimate of 86.6% was calculated for LMA phenotype measured as optical density in a collection of 91 SHWs. Linkage disequilibrium extended up to 10 cM, and with controls for false positives, significant markers were detected at the chromosome 7B region previously linked to LMA in bread wheat, but not at the chromosome 3B region. Of potentially great interest is a region on chromosome 6B, which was identified as having a significant association with LMA phenotypes in the SHW accessions. Previous investigations suggested existence of an LMA gene on the long arm of 6B, but this is the first time it has been mapped to lie within the centromeric region of chromosome 6B, a region that harbours the Amy-1 genes and whose expression governs activity of the high pI α-amylase isoenzymes.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-009-9375-7