Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Objective To investigate the effect of antifreeze protein (AFP) supplementation during mouse oocyte vitrification on the survival, fertilization and embryonic development. Design Animal study. Setting University laboratory. Animal(s) BDF-1 mice. Intervention(s) In vivo–matured metaphase II oocytes w...

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Bibliographic Details
Published in:Fertility and sterility 2011-11, Vol.96 (5), p.1239-1245
Main Authors: Jo, Jun Woo, M.S, Jee, Byung Chul, M.D, Lee, Jung Ryeol, M.D, Suh, Chang Suk, M.D
Format: Article
Language:English
Subjects:
adenosine triphosphate
Adenosine Triphosphate - metabolism
Animals
Antifreeze protein
antifreeze proteins
Antifreeze Proteins - pharmacology
Biological and medical sciences
blastocyst
Blastocyst - drug effects
Blastocyst - metabolism
Blastocyst - pathology
caspases
Cell Membrane - drug effects
Cell Membrane - metabolism
Cell Membrane - pathology
chilling injury
chromosomes
Cryopreservation
Cryoprotective Agents - pharmacology
Diseases of the digestive system
Embryo Culture Techniques
embryogenesis
Female
Fertilization in Vitro
gene expression
Gene Expression Regulation, Developmental - drug effects
Gynecology. Andrology. Obstetrics
Internal Medicine
Medical sciences
metaphase
Mice
Obstetrics and Gynecology
oocyte
oocytes
Oocytes - drug effects
Oocytes - metabolism
Oocytes - pathology
protective effect
protein supplements
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
spindle
Vitrification
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Summary:Objective To investigate the effect of antifreeze protein (AFP) supplementation during mouse oocyte vitrification on the survival, fertilization and embryonic development. Design Animal study. Setting University laboratory. Animal(s) BDF-1 mice. Intervention(s) In vivo–matured metaphase II oocytes were vitrified with the use of CryoTop by two-step exposure to equilibrium and vitrification solution supplemented or not with 500 ng/mL AFP III. Main Outcome Measure(s) Postwarming survival, fertilization, embryonic development up to blastocyst in vitro, morphology of spindle and chromosome, membrane integrity, adenosine triphosphate (ATP) contents, and several gene expressions. Result(s) In the AFP-treated group, blastocyst formation rate was significantly higher and blastomere count with positive caspase was significantly lower compared with the nontreated group. Rate of intact spindle/chromosome, stable membrane, and ATP contents were significantly higher in AFP group. AFP group showed higher Mad2 and lower Eg5 gene expression. Both vitrification groups showed increased Hsf1 , Zar1, and Zp1 / Zp2 expression and decreased Hook1 and Zp3 expression compared with fresh control samples. Conclusion(s) Supplementation of AFP in vitrification medium has a protective effect on mouse oocytes for chilling injury; it can preserve spindle/membrane integrity and intracellular ATP contents. More stable spindle integrity in the AFP group may be associated with higher Mad2 and lower Eg5 gene expression.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2011.08.023