Passive immunisation against Pseudomonas aeruginosa recombinant flagellin in an experimental model of burn wound sepsis

Abstract Background This study was aimed to investigate whether anti-recombinant flagellin type A (anti r-fla-A) immunoglobulin G (IgG) provides protection in a mouse burn model of infection, and to determine the role of anti r-fla-A IgG as an opsonin and motility inhibitor in local and systemic inf...

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Bibliographic Details
Published in:Burns 2011-08, Vol.37 (5), p.865-872
Main Authors: Faezi, Sobhan, Sattari, Morteza, Mahdavi, Mehdi, Roudkenar, Mehryar Habibi
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - therapeutic use
Bacterial diseases
Bacterial sepsis
Biological and medical sciences
Blotting, Western
Burns
Burns - drug therapy
Burns - microbiology
Critical Care
Flagellin - immunology
Human bacterial diseases
Immunisation
Immunization, Passive
Immunoglobulin G - immunology
Infectious diseases
Medical sciences
Mice
Passive
Phagocytosis - drug effects
Prevention and actions
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas Infections - immunology
Pseudomonas Infections - mortality
Pseudomonas Infections - prevention & control
Public health. Hygiene
Public health. Hygiene-occupational medicine
Recombinant flagellin
Recombinant Proteins - therapeutic use
Sepsis - drug therapy
Sepsis - microbiology
Sepsis - prevention & control
Skin - microbiology
Traumas. Diseases due to physical agents
Wound Infection - drug therapy
Wound Infection - microbiology
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Summary:Abstract Background This study was aimed to investigate whether anti-recombinant flagellin type A (anti r-fla-A) immunoglobulin G (IgG) provides protection in a mouse burn model of infection, and to determine the role of anti r-fla-A IgG as an opsonin and motility inhibitor in local and systemic infections. Methods Following the preparation of r-flagellin type A, rabbit polyclonal IgG was prepared. Specificity of anti r-flagellin for r-flagellin was evaluated by immunoblot analysis. After burn and challenge, mortality rate was screened in the mice treated with anti r-fla-A IgG. The ability of antiserum to promote phagocytosis of bacteria was assessed by the opsonophagocytosis testing. Functional activity of anti r-fla-A IgG was assessed in vitro by motility inhibition assay. Bacterial quantity in skin and internal organs was evaluated to study systemic infection. Results In vivo administration of anti r-fla-A IgG resulted in a significant improvement in survival in mice infected by a homologous strain of Pseudomonas aeruginosa from 16.6% to 75% compared with the control IgG. By contrast, this rate was 33.3% in the mice infected by the heterologous strain, PAO1 (type B flagellated strain). Protection was improved by giving a second treatment of r-flagellin antisera at 24-h post-burn and infection. Furthermore, anti r-fla-A IgG enhanced considerably the phagocytosis of the homologous strain but it was slight in the heterologous strain. The antiserum against r-flagellin type A was able to inhibit the motility of the PAK strain (type A flagellated strain), but slight inhibition was observed against PAO1. Meanwhile, anti r-fla-A IgG inhibited the systemic spread of PAK strain from the site of infection to internal organs. Conclusion In this study, passive immunisation with anti r-fla-A IgG was active against a homologous strain of infecting P. aeruginosa , but lost most of its efficiency against a heterologous strain. Therefore, passive treatment with anti r-fla-A IgG might protect burned mice against local and systemic infection of P. aeruginosa.
ISSN:0305-4179
1879-1409
DOI:10.1016/j.burns.2010.12.003