Hydrocortisone nanosuspensions for ophthalmic delivery: A comparative study between microfluidic nanoprecipitation and wet milling

Recently, drug nanosuspensions have shown a potential for ophthalmic delivery. In this study, a hydrocortisone (HC) nanosuspension (NS) was developed using microfluidic nanoprecipitation as a recent, simple and cost-effective bottom-up technique of drug nanonization. For comparison, a second HC NS w...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2011-01, Vol.149 (2), p.175-181
Main Authors: Ali, Hany S.M., York, Peter, Ali, Ahmed M.A., Blagden, Nicholas
Format: Article
Language:English
Subjects:
Animals
bioavailability
Biological and medical sciences
Biological Availability
Chemical Precipitation
Controlled release
cortisol
cost effectiveness
crystal structure
differential scanning calorimetry
Drug Carriers - chemistry
Drug Compounding - methods
drugs
Eye - metabolism
General pharmacology
Hydrocortisone
Hydrocortisone - administration & dosage
Hydrocortisone - pharmacokinetics
Male
Medical sciences
Microfluidics
Milling
Nanostructures - chemistry
Nanosuspension
Nanotechnology - methods
Ophthalmic delivery
Ophthalmic Solutions
Particle Size
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Rabbits
Solutions
Suspensions
temperature
wet milling
Wettability
X-ray diffraction
zeta potential
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, drug nanosuspensions have shown a potential for ophthalmic delivery. In this study, a hydrocortisone (HC) nanosuspension (NS) was developed using microfluidic nanoprecipitation as a recent, simple and cost-effective bottom-up technique of drug nanonization. For comparison, a second HC NS was prepared by top-down wet milling procedures. The produced nanosuspensions were characterized for particle size, shape and zeta potential. HC nanosuspensions of approximately 300 nm particle size were produced by adjusting experimental conditions of the two processing techniques. Results of X-ray diffraction and differential scanning calorimetry revealed that HC maintained the crystalline structure upon milling, while predominant amorphous particles were generated after precipitation. Ocular bioavailability of HC nanosuspensions was assessed in albino rabbits using HC solution as a control. A sustained drug action was maintained up to 9 h for the nanosuspensions compared to 5 h for the drug solution. The precipitated and milled NS achieved comparable AUC 0–9h values of 28.06 ± 4.08 and 30.95 ± 2.2, respectively, that were significantly ( P < 0.05) higher than that of HC solution (15.86 ± 2.7). After 2 months storage at room temperature, the milled HC NS showed good stability with no discernable changes in particle size, whereas the particle size of the precipitated HC NS increased to 440 nm. Changes in intraocular pressure (IOP) of Albino rabbits after administration of Hc solution and nanosuspensions produced by milling and precipitation. [Display omitted]
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2010.10.007