Kinetic characterization and gene expression of adenosine deaminase in intact trophozoites of Trichomonas vaginalis

Abstract Trichomonas vaginalis is a parasite that resides in the human urogenital tract and causes trichomonosis, the most prevalent nonviral sexually transmitted disease. Nucleoside triphosphate diphosphohydrolase (NTPDase), which hydrolyzes extracellular di- and triphosphate nucleotides, and ecto-...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2011-06, Vol.319 (2), p.115-124
Main Authors: Weizenmann, Marina, Frasson, Amanda Piccoli, de Barros, Muriel Primon, Vieira, Patrícia de Brum, Rosemberg, Denis Broock, De Carli, Geraldo Attilio, Bogo, Maurício Reis, Bonan, Carla Denise, Tasca, Tiana
Format: Article
Language:English
Subjects:
Adenine
Adenosine
Adenosine deaminase
Adenosine Deaminase - chemistry
Adenosine Deaminase - genetics
Adenosine Deaminase - metabolism
AMP
Anti-inflammatory agents
Biological and medical sciences
Calcium
Deamination
Dictyostelium discoideum
Disease transmission
Entamoeba histolytica
Ethylenediaminetetraacetic acids
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Enzymologic
Gene sequencing
Genomes
Homology
Humans
Inflammation
inosine
intact trophozoites
Kinetics
Magnesium
Microbiology
Nucleosides
Nucleotidase
Nucleotides
Parasitic diseases
Phylogeny
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
RNA-directed DNA polymerase
Sexually transmitted diseases
STD
Trichomonas vaginalis
Trichomonas vaginalis - chemistry
Trichomonas vaginalis - enzymology
Trichomonas vaginalis - genetics
Trichomonas vaginalis - growth & development
Trichomonas Vaginitis - parasitology
Trophozoites
Trophozoites - chemistry
Trophozoites - enzymology
Trophozoites - growth & development
Tubulin
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Trichomonas vaginalis is a parasite that resides in the human urogenital tract and causes trichomonosis, the most prevalent nonviral sexually transmitted disease. Nucleoside triphosphate diphosphohydrolase (NTPDase), which hydrolyzes extracellular di- and triphosphate nucleotides, and ecto-5′-nucleotidase, which hydrolyzes AMP, have been characterized in T. vaginalis. The aim of this study was to characterize the adenosine deaminase (ADA) activity in intact trophozoites of T. vaginalis. A strong inhibition in adenosine deamination was observed in the presence of calcium and magnesium, which was prevented by EDTA. The apparent KM value for adenosine was 1.13 ± 0.07mM. The calculated Vmax was 2.61 ± 0.054nmolNH3min−1mg−1protein. Adenosine deamination was inhibited in the presence of erythro-9-(2-hydroxy-3-nonyl)adenine. Semi-quantitative reverse transcriptase-PCR experiments were performed and both ADA-related genes ada(125) and ada(231) mRNA were expressed, although ada(231) in higher quantity when compared with the ada(125):α-tubulin ratio. Furthermore, a phylogenetic analysis showed that the T. vaginalis sequences formed a clade with Entamoeba histolytica and Dictyostelium discoideum sequences, and it strongly suggests homologous functions in the T. vaginalis genome. The presence of ADA activity in T. vaginalis may be important to modulate the adenosine/inosine levels during infection and, consequently, to maintain the anti-inflammatory properties through different nucleoside-signalling mechanisms.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2011.02283.x