Radiosensitizing effect of ferulic acid on human cervical carcinoma cells in vitro

► Adjuvant for cancer radiotherapy. ► Dietary natural phenolics for radiosensitisation. ► Ferulic acid exhibits prooxidant property in cancer cells. ► Ferulic acid augmenting radiation effect in cervical carcinoma cells. Radiotherapy may be effectively combined with plant derived radiosensitizers. F...

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Bibliographic Details
Published in:Toxicology in vitro 2011-10, Vol.25 (7), p.1366-1375
Main Authors: Karthikeyan, Subburayan, Kanimozhi, Govindhasamy, Prasad, Nagarajan Rajendra, Mahalakshmi, Rajendran
Format: Article
Language:English
Subjects:
Antioxidants
Antioxidants - metabolism
Apoptosis - drug effects
Apoptosis - radiation effects
Carcinoma - drug therapy
Carcinoma - radiotherapy
Cell Line, Tumor
Cervical cancer
Coumaric Acids - chemistry
Coumaric Acids - pharmacology
DNA Damage - drug effects
DNA Damage - radiation effects
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Female
Ferulic acid
HeLa Cells
Humans
Inhibitory Concentration 50
Lipid Peroxidation
Molecular Structure
Olea
Radiation
Radiation-Sensitizing Agents - chemistry
Radiation-Sensitizing Agents - pharmacology
Radiosensitization
Reactive Oxygen Species - metabolism
ROS
Uterine Cervical Neoplasms - drug therapy
Uterine Cervical Neoplasms - radiotherapy
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Summary:► Adjuvant for cancer radiotherapy. ► Dietary natural phenolics for radiosensitisation. ► Ferulic acid exhibits prooxidant property in cancer cells. ► Ferulic acid augmenting radiation effect in cervical carcinoma cells. Radiotherapy may be effectively combined with plant derived radiosensitizers. Ferulic acid, a naturally occurring phenolic acid, has been reported to have free radical producing properties. In the present study, the radiosensitisation potential of ferulic acid has been tested in two cervical cancer cell lines (HeLa and ME-180) in vitro. Percentage of growth inhibition (MTT assay), colony survival, levels of lipid peroxidation (TBARS, CD and LHP), antioxidant status (SOD, CAT, GPx and GSH), oxidative DNA damage (% tail DNA, tail length, tail moment and Olive tail moment), apoptotic morphological changes (AO/EtBr staining) and intracellular ROS levels (DCFH-DA) were estimated. The present results show that ferulic acid (FA) enhances radiation effects by increasing lipid peroxidative markers in HeLa and ME-180 cells. We observed significant enhancement of ROS levels during ferulic acid plus radiation treatment. FA treatment alone increased intracellular ROS levels indicate its prooxidant nature. Similarly, we observed enhanced oxidative DNA damage and apoptotic morphological changes in FA plus radiation treated cells. The present data suggest radiation sensitizing property of FA in cervical cancer cells. Further investigations warrants to substantiate the present findings.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2011.05.007